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URL: http://www.addgene.org/42593
Proper Citation: RRID:Addgene_42593
Insert Name: hairpin - BCL2 5'UTR - firefly luciferase
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15123638
Vector Backbone Description: Backbone Marker:Stanley Lemon (PubMed ID 10611164); Vector Backbone:pRL-HL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: phpBCL2-FL(pA) includes a 143-bp stable RNA hairpin located upstream of BCL2 5'UTR and firefly luciferase. See plasmid map link above for more details. This plasmid is one of several reporter constructs created to assess potential IRES activity of the BCL2 5'UTR. The hairpin in this construct inhibits the ability of the BCL2 5'UTR to mediate translation of firefly luciferase. Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.
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Source: Addgene
