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URL: http://www.addgene.org/128253
Proper Citation: RRID:Addgene_128253
Insert Name: Beta Globin Intron::hTetR::P2A::EGFP
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:31235692
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:7505; Vector Backbone:pDN-D2irTNG5kwh; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Comments: The plasmid encodes a mammalian synthetic gene circuit with the humanized Tetracycline Repressor (hTetR) regulator separated from EGFP by a P2A motif, which is controlled by a CMV-derived promoter containing 2 tetO2 sites. With hTetR binding to the promoter, negative feedback lowers gene expression noise while linearizing the mean dose response. The circuit is also called mNF-GFP and is within a Flp-In expression vector.
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Source: Addgene