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URL: http://www.addgene.org/12570
Proper Citation: RRID:Addgene_12570
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16919458
Vector Backbone Description: Backbone Marker:Modified from Clontech's pMSCV; Backbone Size:6800; Vector Backbone:pMSCV-TAP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5' -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3' and right primer 5' -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3' . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clontech) cut with BglII/EcoRI. See author's map for more information (Note that the NotI site is not precisely underlined in the author's map).
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Source: Addgene
