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URL: http://www.addgene.org/11238
Proper Citation: RRID:Addgene_11238
Insert Name: IRS-1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10958681
Vector Backbone Description: Backbone Size:7583; Vector Backbone:pCMV histidinol; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: pCMV human IRS-1 was cut with ApaLI and SalI. A 5' IRS-1 oligo at bp4746 (amino acid 1242) and 3'IRS-1 oligo with 3' IRS-1 sequence fused to the p21ras CAAX prenylation motif (CMSCKCVLS) were used for PCR and the insert was ligated into pCMV human IRS-1. Select cells expressing plasmid with approximately 5mM histidinol (Sigma), depending on the cell type.
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Source: Addgene
