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URL: http://www.addgene.org/78204
Proper Citation: RRID:Addgene_78204
Insert Name: ssb (W88C)
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20350930
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note- The numbering system used was for the mature natural wild-type ssb protein. The N-terminal methionine is quantitatively cleaved off in E. coli and was not numbered so all mutations will be off by 1 nucleotide (e.g., W89C = W88C). The expressed protein is a single-cysteine variant of E. coli single-stranded DNA-binding protein, EcSSB, for subsequent labeling at the cysteine. The adduct with Cy3B maleimide has been used as a biosensor for single-stranded DNA in TIRF microscopy measurements.
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Source: Addgene
