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URL: http://www.addgene.org/78199
Proper Citation: RRID:Addgene_78199
Insert Name: pstS (A17C,A197C)
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26199994
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note- The numbering system used was for the mature natural wild-type PstS protein. The plasmid produces an extra N-terminal methionine so all mutations will be off by 1nt (e.g., A197C =A198C) The expressed protein is a double-cysteine variant of E. coli phosphate binding protein. The gene encodes the mature protein without the N-terminal signal peptide, but with an N-terminal methionine, for subsequent labeling at the cysteines with two 6-iodoacetamidotetramethylrhodamines per molecule. This adduct is then used as a fluorescent phosphate biosensor, having 10-15-fold fluorescence increase on binding inorganic phosphate with very high affinity.
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Source: Addgene
