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URL: http://www.addgene.org/71461
Proper Citation: RRID:Addgene_71461
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16074986
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the CAT gene, which can be replaced with your gene of interest using the unique BsrG I site (TGTACA) at the end of the intein and one of several sites downstream from the product protein (typically Hind III). More info on cloning can be found in supplemental document ELP cloning manual. Please NOTE- the Addgene diagnostic digest result confirms the presence of ELP-intein.
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Source: Addgene
