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URL: http://www.addgene.org/71383
Proper Citation: RRID:Addgene_71383
Insert Name: human synapsin promoter-flex switch-dsRed-shscramble
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26094607
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: this plasmid is modified from the pRIME system. See: Stegmeier, F., Hu, G., Rickles, R.J., Hannon, G.J., and Elledge, S.J. (2005). A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc. Natl. Acad. Sci. USA 102, 13212–13217 We used The pPRIME-CMV-dsRed-FF3 vector, a gift from Stephen Elledge (Addgene plasmid 11664), as the building block to make the AAV-hsyn-flex-dsRed-shscramble plasmid All the enzymes are unique cutters, except EcoRI and XhoI, the insert dsRed-shRNA can be retrieved using AscI and NheI, giving 1.2kb and 4.8kb bands. The insert was read using primers in the dsred sequence: dsred 410: CGTAATGCAGAAGAAGACTATG dsred 530R: CCATGTAGATGGACTTGAACTC
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Source: Addgene
