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URL: http://www.addgene.org/46263
Proper Citation: RRID:Addgene_46263
Insert Name: Vcl 1-258
Organism: Gallus gallus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15728584
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: EYFP was PCR amplified to introduce NdeI site at both 5' and 3' ends. The NdeI flanked EYFP was inserted into NdeI site of pET15b/Vh1-258 (Addgene plasmid 46173), where V1-258 was cloned between NdeI and XhoI. Plasmid contains a 12-residue spacer between the EYFP and vinculin domain.
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Source: Addgene
