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URL: http://www.addgene.org/42595
Proper Citation: RRID:Addgene_42595
Insert Name: hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15123638
Vector Backbone Description: Backbone Marker:Stanley Lemon (PubMed ID 10611164); Vector Backbone:pRL-HL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: phpRL-BCL2-FL(pA) includes a 143-bp stable RNA hairpin located upstream of renilla luciferase, BCL2 5'UTR and firefly luciferase. See plasmid map link above for more details. This plasmid is one of several reporter constructs created to assess potential IRES activity of the BCL2 5'UTR. The hairpin in this construct inhibits translation of renilla luciferase and to a lesser extent translation of firefly luciferase. Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.
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Source: Addgene
