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Plasmid Name
RRID:Addgene_42209 RRID Copied  
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RRID:Addgene_42209
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Plasmid Information

URL: http://www.addgene.org/42209

Proper Citation: RRID:Addgene_42209

Insert Name: c-SRC (250-536) HA

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:10753943

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: All c-SRC mutants were generated by polymerase chain reaction using c-SRC (WT) (Addgene Plasmid #42202) as a template, and polymerase chain reaction products containing a minimal Kozak sequence were cloned into the HindIII and XhoI sites of pcDNA3. This construct contains amino acids 250-536 of human c-SRC followed by a carboxyl-terminal HA tag. All sequences were confirmed using an automated ABI DNA sequencer (Howard Hughes Nucleic Acid Facility, Duke University). Alternate plasmid name: pcDNA3 c-SRC-SH1

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Data and Source Information

Source: Addgene