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URL: http://www.addgene.org/42196
Proper Citation: RRID:Addgene_42196
Insert Name: β-arrestin 1 S412D
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9924018
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing.
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Source: Addgene
