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URL: http://www.addgene.org/27989
Proper Citation: RRID:Addgene_27989
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21091446
Vector Backbone Description: Backbone Marker:Jie Yang Lab; Backbone Size:3300; Vector Backbone:pLIC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is used for a novel and straightforward ligation-independent cloning (LIC) method based on nicking DNA endonuclease (NiDE). To construct a NiDE-compatible pLIC vector, LacZ cDNA was amplified from a modified pMD18 construct by PCR using primers Laz-BgiII-F and Laz-EcoR I-R. The β-galactosidase gene lacZ, including restriction endonuclease sites such as BamHI, SalI, PstI, and SphI, was amplified and then digested by BglII and EcoRI. It was then cloned into pRSETb plasmid between BamHI and EcoRI sites to generate the pLIC vector. See Author's Map and published article for more information.
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Source: Addgene
