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URL: http://www.addgene.org/246943
Proper Citation: RRID:Addgene_246943
Insert Name: Type IF CRISPR-Cas system from Pseudomonas aeruginosa PA14
Organism: Pseudomonas aeruginosa PA14
Bacterial Resistance: Ampicillin and Gentamicin
Defining Citation: PMID:40904105
Vector Backbone Description: Backbone Marker:Herbert Schweizer (Addgene #63121); Backbone Size:4500; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Gentamicin
Comments: Synthetic Type IF CRISPR-Cas system based on the sequence from Pseudomonas aeruginosa PA14 with a minimised version of the CRISPR 2 array containing a non-targeting spacer with BbsI restriction sites for cloning. Native promoter regions were cloned as the intergenic region between the CRISPR components and upstream genes. MluI, NsiI, NotI, NcoI, AscI restriction sites were added to make the system modular.
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Source: Addgene
