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URL: http://www.addgene.org/192872
Proper Citation: RRID:Addgene_192872
Insert Name: MG1655-C321 mutS+, λ-, Δ(ybhB-bioAB)::zeoR, ΔprfA, ΔserB
Organism: E. coli
Bacterial Resistance: Bleocin (Zeocin)
Defining Citation: PMID:36280721
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Comments: MG1655-C321 -- All 321 UAG codons changed to UAA. mutS+ -- Restores normal spontaneous mutation frequency. (λ-) – Deleted Lambda Phage operon to allow growth at 37C. Δ(ybhB-bioAB)::zeoR – Deletion of biotin biosynthetic operon using Zeocin cassette; strain is d-biotin auxotroph. ΔprfA – Deletion of polypeptide release factor 1 (RF1) to prevent translation termination at UAG codons. ΔserB – Deletion of phosphoserine phosphatase to provide intracellular source of phosphoserine; strain is Serine auxotroph. The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1)
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Source: Addgene
