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Plasmid Name
RRID:Addgene_178877 RRID Copied  
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RRID:Addgene_178877
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Plasmid Information

URL: http://www.addgene.org/178877

Proper Citation: RRID:Addgene_178877

Insert Name: wrmScarlet11^SEC^3xMyc

Organism: Caenorhabditis elegans

Bacterial Resistance: Ampicillin

Vector Backbone Description: Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin

Comments: The split-wrmScarlet system was developed and published by Goudeau et al. Genetics 2021. pGLOW63 is a derivative of published TagRFP-SEC vectors (Dickinson et al. Genetics 2015). It has a 3xMyc tag in place of 3xFlag and Lox2272 sites in place of LoxP. These features allow pGLOW63 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites. pGLOW63 must also be used in a genetic background that has wrmScarlet1-10. Made by Amelie Perez (Glow Worms '21).

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Data and Source Information

Source: Addgene