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URL: http://www.addgene.org/127536
Proper Citation: RRID:Addgene_127536
Insert Name: Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
Organism: Other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32444777
Vector Backbone Description: Vector Backbone:pBluescript II SK(-); Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original Bxb1 coding sequence (before A. thaliana codon optimization) was obtained from Bonnet, J. et al. Science 340, 599-603 (2013) (Genbank KC529324.1).
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Source: Addgene
