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URL: http://www.addgene.org/126754
Proper Citation: RRID:Addgene_126754
Insert Name: eSpCas9
Organism: S. pyogenes
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32144253
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: pCbh-3xFLAG-NLS-eSpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations). The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457). For detailed information and plasmid usage, please see the associated publication.
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No rating or validation information has been found for pX330-Flag-eSpCas9 (without sgRNA; with silent mutations).
No alerts have been found for pX330-Flag-eSpCas9 (without sgRNA; with silent mutations).
Source: Addgene
