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URL: http://www.addgene.org/126643
Proper Citation: RRID:Addgene_126643
Insert Name: LbCas12a Full Length Direct Repeat GC Swap crRNA
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30892626
Vector Backbone Description: Backbone Size:3232; Vector Backbone:pBCSK+; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: For cloning crRNA guide sequences into the GC swap frameworks: The top oligo should contain a GTGG overhang on its 5' end and the bottom oligo should contain AAAA overhang on its 5' end to match the overhangs that are produced by BbsI. In addition, for these constructs the guide oligos should encode the final two bases [GT] of the constant region of the crRNA prior to the spacer [targeting] sequence and then two additional Ts following the spacer sequence to ensure robust RNA Polymerase III termination.
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No rating or validation information has been found for LbCas12a-DR-GC-swap-cr in pBCSK+.
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Source: Addgene
