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URL: http://www.addgene.org/124540
Proper Citation: RRID:Addgene_124540
Insert Name: delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30940799
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Engineered tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion. Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
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No rating or validation information has been found for 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA.
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Source: Addgene
