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Plasmid Name
pPRISM-gal4-VP16-gcry-mRFP
RRID:Addgene_117778 RRID Copied  
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RRID:Addgene_117778
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Plasmid Information

URL: http://www.addgene.org/117778

Proper Citation: RRID:Addgene_117778

Insert Name: gal4/VP16

Organism: Danio rerio

Bacterial Resistance: Kanamycin

Defining Citation: PMID:32412410

Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin

Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at . gal4-VP16 should be used in conjunction with a UAS expression line. May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function. This plasmid has been found exist in our stock as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

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Data and Source Information

Source: Addgene

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