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URL: http://www.addgene.org/113799
Proper Citation: RRID:Addgene_113799
Insert Name: DEAF1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 62987); Vector Backbone:pX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. This is a nickase guide and must be used with the additional nickase plasmid pDEAF1.2.1-gDNA and donor plasmid pDEAF1-donor.
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Source: Addgene
