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URL: http://www.addgene.org/113762
Proper Citation: RRID:Addgene_113762
Insert Name: tdNfsB(F124W)-mCherry
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30111097
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed."
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No rating or validation information has been found for aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR).
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Source: Addgene
