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URL: http://www.addgene.org/107726
Proper Citation: RRID:Addgene_107726
Insert Name: MAPRE1 gRNA #1 (targets Exon 1)
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29379139
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below) For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3 Generation of cell lines with light-controlled microtubule dynamics Torsten Wittmann & Jeffrey van Haren Protocol Exchange (2018) doi:10.1038/protex.2017.155 https://www.nature.com/protocolexchange/protocols/6427
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No rating or validation information has been found for pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1.
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Source: Addgene
