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Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans.

Lourds M Fernando | Kyrionna Golliday | Ruby Boateng | Anna K Allen
microPublication biology | 2021

We have generated a WEE-1.3 strain in C. elegans wherewe have endogenously tagged the C-terminus with GFP. In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that have been previously published. We also show for the first time that endogenously tagging WEE-1.3 at either termini does not affect the reproductive function of the worms.

Pubmed ID: 33474525

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Associated grants

  • Agency: NICHD NIH HHS, United States
    Id: R15 HD084253

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NIS-Elements (tool)

RRID:SCR_014329

Microscope imaging software suite used with Nikon products. NIS-Elements includes software applications for advanced and standard research, documentation, confocal microscopy, and high-content analysis.

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