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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-2/janA-2; eja1-1/eja1-1 (janA-2; eja1-1; janA, eja1, II)
Affected Genes: JANA (janus A) (originally reported as jan); EJA1 (enhancer of janus)
Source References: PMID:1743393
Notes: A mutation that causes the dorsal surface to be converted to a partial mirror-image of the ventral surface and blocks conjugation. Allelic to janA-1.
Proper citation: RRID:TSC_SD00643 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Affected Genes: (The calmodulin IES)
Source References: PMID:15037070
Notes: Submitted by P. Huvos, Southern Illinois Univesity
Proper citation: RRID:TSC_SD01734 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: fat2-1/fat2-1 (fat2-1, II)
Affected Genes: FAT2 (formerly FATB)
Notes: Somewhat pudgy but otherwise completely normal. Expression is not appreciably temperature-sensitive.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01221 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Proper citation: RRID:TSC_SD00890 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 2R [B or C3] (pmr1-1; cnj3, pm-r, VII)
Proper citation: RRID:TSC_SD00379 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, A101G)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations A101G and c-terminus HA tag into the MTT1 locus, partial replacement.
Source References: PMID:12356864, PMID:16344310
Notes: pGTUA101G-HA rescued GTUKO 5x6 progeny having mutation @ GTU1 A101G in GTU1-1 gene, driven by MTT1 promoter.
Grow and maintain in 0.5 ug/ml CdCl2 at 30C.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02043 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-1/hpo1-1; janC-1/janC-1 (hpo 1-1; janC-1; hpo1; janC, IV)
Affected Genes: HPO1 (hypoangular 1), JANC (janusC)
Notes: janC is very weakly expressed in this double homozygote.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01751 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1
(mtt1-?[5’neo2,∆::BLT1, HAc], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’flanking region
Notes: CU428 somatic replacement with BLT-HA tag in the MTT1 locus, has Neo2 in 5’ flanking region of MTT1. Wild type BLT1 gene still present. This is a partial replacement. Cannot single-cell, only some cells express the HA tagged BLT when induced with Cd.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02563 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: presumed phg-/phg- (presumed phg-; phg-, ?)
Source References: PMID:23195323, PMID:18658256, PMID:18311715
Notes: The Frankel lab could not obtain progeny from crosses using this stock. Its observations are consistent with the reported temperature-sensitive food-vacuoleless phenotype, but not consistent with the reported total loss of oral structures. This stock exhibits multiple unique morphogenetic phenotypes at 37.5o C.: see Frankel (2008: p. 1626, 1632). The Iowa stock is designated II8G-IA to distinguish it from the II8G stock, deposited by L. Rasmussen at the American Type Culture Collection. ATCC.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01631 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆HTB1/∆HTB1 pm-r with neo2 (pm-r, cy-s)
Affected Genes: HTB1 (TTHERM_00633360)
Notes: HTB1 complete KO strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01999 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆HHT1/∆HHT1; ∆HHT3/∆HHT3
hht1[∆::neo2]/hht1[∆::neo2];hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT1 + HHT3 (TTHERM_00570560, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT3
Source References: PMID:16908532
Notes: homozygous, Neo cassette knockout
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01879 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mrp1-1/mpr1-1 (twi1[3’neo3, FLAG-HAn]; pm-r, VII)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: FLAG-HA tagged TWI1 with neo3 replaced all TWI1
Source References: PMID:12297043
Notes: All somatic TWI1 was replaced by FLAG-HA-TWI1 construct. CU428 background.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02609 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (CNA1/cna1[∆::neo2]; pm-r, VII)
Affected Genes: CNA1 (TTHERM_00146340)
Genomic Alteration: Macronucleus: neo2 into CNA1
Source References: PMID:16738316
Notes: CNA1 KO construct is transformed inWT mac, assorted to 500ug/ml pm resistance. Complete replacement not possible, gene is essential.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02693 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (Circular permutation of HHO1 E5 charge patch, impossible to define in correct nomenclature, see paper.; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Source References: PMID:11972045
Notes: Somatic transformation of CU428 with HHO1 construct E-C
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02294 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mp-r, ?)
Affected Genes: HHT2 + HHT3 (TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT3 and HHT2/HHF2 gene with its intergenic region
Source References: PMID:16908532
Notes: This is the non-star side of round I genomic exclusion. It should have the same mic genome as ∆H3.2/3.3 gl KO 1*strain
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01880 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (twi1[3’neo3, FLAG-HA]; FLAG-HA TWI1, pmr (neo3), VII)
Affected Genes: TWI1
Genomic Alteration: Macronucleus: FLAG-HA tagged TWI1 with neo3 replaced all TWI1
Source References: PMID:12297043
Notes: All somatic TWI1 was replaced by FLAG-HA-TWI1 construct. CU428 background.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02613 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (?HHT1/hht1[∆::neo2; hht2[3’neo2, S10A]; Somatic ∆HHT1 with neo cassette and HHT2S10A ( neo in 3 flank) cotransformant., VI)
Affected Genes: HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: KO of HHT1, HHT2 has mutation S10A
Source References: PMID:10199406, PMID:9636175
Notes: Single cell line from H3E transformant.
By antiphos H3 antibody staining, no stain so should be all HHT2 S10A. Also checked by Southern.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01889 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Proper citation: RRID:TSC_SD00832 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[∆?::neo3]/twi11[∆?::neo3] (mpr1-1, TWI11)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: Part of TWI11 coding replaced with neo3 cassette, homozygous
Notes: Homozygous TWI11 KO heterokaryon partial coding sequence in MIC were replaced with neo3 cassette
.
mates withTWI11 KO1-4-R1
This strain was supposed to be mp-r, but thawed mp-s.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02857 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; MPR1[C3]/MPR1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];MPR1[C3]; pmr1-1; MAT2; cy-s, mp-s, pm-r, ?)
Source References: PMID:8725229, PMID:8601476
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01252 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
