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SciCrunch Registry is a curated repository of scientific resources, with a focus on biomedical resources, including tools, databases, and core facilities - visit SciCrunch to register your resource.
https://www.nanostring.com/products/ncounter-analysis-system/ncounter-systems-overview/
System includes hardware and software. Options include nCounter SPRINT,nCounter MAX or nCounter FLEX. Platform used for multiplex analysis of up to 800 RNA, DNA, or protein targets. System can be combined with GeoMx Digital Spatial Profiler (DSP) to enable high-plex, spatially-resolved RNA, and protein quantification.
Proper citation: NanoString: nCounter Analysis System (RRID:SCR_021712) Copy
https://www.ohsu.edu/transgenic-mouse-models-core
Core assists investigators with developing genetically engineered rodent models of human diseases for studying mutant genes and investigating molecular mechanisms underlying pathological processes.
Proper citation: OHSU Animal Model Support Core Facility (RRID:SCR_009994) Copy
Center that imports, archives, maintains, and distributes mutant mouse alleles as live mice, frozen germplasm, stem cells, and molecular vectors for use in biomedical research. The MMRRC Davis receives transgenics, knockouts, and other kinds of mutant mouse lines at no cost to the donor, and after re-derivation and cryopreservation, distributes breeding stock, germplasm, cells, or tissues of genetically-defined and pathogen-free mice for a small fee to requesting investigators.
Proper citation: University of California at Davis Mutant Mouse Resource and Research Center (RRID:SCR_016448) Copy
https://www.rankinbiomed.com/product/refurbished-american-optical-860-sliding-micrtome/
THIS REOURCE IS NO LONGER IN SERVICE. Documented on September 29,2023. Sliding microtome used for cutting hard objects such as bone, wood, frozen celloidin, and paraffin preparations. Cutting thickness is adjustable from 1 to 40 microns.
Proper citation: American Optical: 860 Sliding Microtome (RRID:SCR_018434) Copy
https://atagousa.corecommerce.com/PAL-10S-p358.html
Digital hand held pocket refractometer that measures urine specific gravity. It has measurement temperature of 10 to 35 C and sample volume of 0.3ml.
Proper citation: Atago: PAL-10S Pocket Refractometer (RRID:SCR_018439) Copy
https://www.sutter.com/manuals/P-2000_OpMan.pdf
Microprocessor controlled, CO2 laser based micropipette puller.Default configuration allows fabrication of micropipettes for intracellular recording, patch clamping, microinjection and microperfusion.Can store up to 100 separate programs, each consisting of up to 8 command lines. The five parameters set in each command line allow exquisite control of the micropipette taper geometry. Allows the use of wide range of glass diameters from 0.125 to 1.2 mm.
Proper citation: Sutter: P-2000 Laser Micropipette Puller (RRID:SCR_018640) Copy
http://tools.thermofisher.com/content/sfs/manuals/nd-1000-v3.8-users-manual-8%205x11.pdf
Spectrophotometer for measurement and analysis of 1 ul samples with high accuracy and reproducibility. Full spectrum from 220nm to 750nm spectrophotometer utilizes patented sample retention technology that employs surface tension alone to hold sample in place. No need for cuvettes. Has capability to measure highly concentrated samples without dilution.
Proper citation: Thermo Fisher: Nanodrop 1000 Spectrophotometer (RRID:SCR_016517) Copy
https://www.thermofisher.com/order/catalog/product/3730XL#/3730XL
96-capillary DNA analyzer that performs DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Has multiple automation features. Performs sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.
Proper citation: Thermo Fisher: Applied Biosystems: 3730xl Capillary Genetic Sequencer (RRID:SCR_018059) Copy
https://www.bio-rad.com/en-us/product/cfx96-touch-real-time-pcr-detection-system?ID=LJB1YU15
Real Time PCR detection system that combines optical technology with temperature control. Six channel, five colors and one FRET channel, real-time PCR instrument for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on integrated LCD touch screen, or use included CFX Maestro Software to design experiment and analyze results from connected computer.
Proper citation: Bio-Rad: CFX96 Real-Time PCR Detection System (RRID:SCR_018064) Copy
https://www.selectscience.net/products/zeiss-lsm-710-for-fluorescence-imaging/?prodID=84549#tab-2
Inverted microscope that enables confocal microscopy in cell and developmental biology.
Proper citation: Zeiss: LSM 710 Confocal Inverted Microscope (RRID:SCR_018063) Copy
https://www.biocompare.com/Product-Reviews/41153-LSM-510-META-From-Zeiss/
Versatile laser scanning confocal microscope system that is available in both upright or inverted format. Equipped with ICS-Optics. Selection of dry, water immersion and oil immersion objectives are available. ICS-Optics are infinity color corrected.
Proper citation: Zeiss: LSM 510 Confocal Microscope (RRID:SCR_018062) Copy
https://www.sonybiotechnology.com/us/instruments/sh800s-cell-sorter/
Benchtop cell sorter that sorts wide range of cell sizes for many applications using microfluidic sorting chips. Integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis. System software supports sorting into tubes and 96- and 384-well plates. Software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. For non-clinical research use only. For applications in which cross-contamination is concern, optional e-beam sterilized consumables can be used to replace sample line and sorting chip. Optional class A2 Level II biosafety cabinet provides protection for personnel and products. Biosafety cabinet meets international standards.Optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection.
Proper citation: Sony biotechnology: SH800S Cell Sorter (RRID:SCR_018066) Copy
https://www.ohio.edu/cas/genomics
Core provides equipment and services.Equipment available includes AB 3130xl Genetic Analyzer 16 capillary array,Nanodrop 1000 Agilent 2100 Bioanalyzer, Agilent 3100 Off Gel Fractionator, Sage Science Pippin Prep, Aria Mx Real Time PCR machine, Illumina MiSeq, Ion Torrent PGM. Research services include DNA sequencing, fragment and microsatellite analysis, AFLP, SNP screening RNA/DNA quantification, quality control, size distribution, RIN analysis, Protein fraction sizing and quanitification, QA/QC purified protein/antibodies, Low cell consumption two-color flow cytometry analysis, Isoelectric focusing prior to Mass Spec, DIGE analysis, or SDS-PAGE, Automated nucleic acid separation and size collection (90 bp to 1.5 kbp), Relative and Absolute qPCR quantification (SYBR, TaqMan probe), End-point PCR genotyping, fragment melt analysis, copy number determination. Provides Next-Generation Sequencing including total RNA, small RNA, exosome and rRNA-depleted RNAseq, SHAPE-seq, 5-end mapping, de novo genome sequencing and targeted DNA sequencing, ChIP-seq, Methyl-seq, Bioinformatic analysis of NGS data, Custom experimental design, optimization, and consulting. Educational services include Hands on training for any basic lab technique or service used at OUGF, Classroom and research lab lectures and informational seminars, Class and small group tours of facility.
Proper citation: Ohio University Genomics Core Facility (RRID:SCR_018268) Copy
Core provides Nextgen sequencing, Microarray, PCR and Bioinformatic services.Offers advanced analysis of microarray data. Provides free initial consultation to discuss project and offers several tiers of analysis packages to best suit your needs. NGS equipment includes NovaSeqs, Seqwell II, Oxford. Microarray expression and genotyping - all platforms.
Proper citation: Washington University School of Medicine Genome Technology Access Center Core Facility (RRID:SCR_018300) Copy
THIS RESOURCE IS NO LONGER IN SERVICE. Documented on November 14,2023.Automated preparation system that isolates and prepares single cell templates for analysis. It can analyze mRNA and DNA sequencing, epigenetics or miRNA expression. It can extract, reverse transcribe, amplify, and analyze gene expression.
Proper citation: Fluidigm: C1 Single-Cell Automated Preparation System (RRID:SCR_018578) Copy
https://www.marshallscientific.com/Fisher-Scientific-PowerGen-125-Homogenizer-p/fs-pg125.htm
Homogenizer with speeds from 8,000 to 30,000 RPM for volumes of up to 100ml. Homogenizer for production of dispersions and emulsions, plant tissues, human tissues, animal tissues, resins, and pigments.
Proper citation: Thermo Fisher: n125 Homogenizer (RRID:SCR_018576) Copy
https://www.gilson.com/verity-271-hplc-system.html
HPLC liquid handler with midsize bed capacity and fraction collector. It has maximum flow rates up to 200 mL per min, injection loop volumes of 2 uL to 25 mL, and max sample mass of 15 grams per injection.
Proper citation: Gilson: GX-271 HPLC System (RRID:SCR_018579) Copy
EImaging scanner that images fluorescent and radioisotopic samples. It can be used for protein electrophoresis, genomics array analysis, fluorescence, radioisotope detection, and chemiluminescence detection by direct detection method.
Proper citation: Fujifilm: FLA-5100 Scanner (RRID:SCR_018581) Copy
https://www.agilent.com/cs/library/brochures/5990-5596EN.pdf
Agilent 3100 OFFGEL Fractionator performs isoelectric focusing of proteins or peptides in immobilized pH gradient (IPG) gel strips.
Proper citation: Agilent: 3100 OFFGEL Fractionator (RRID:SCR_018433) Copy
https://mobile.labwrench.com/equipment/8044/applied-biosystems/voyager-de-pro
Benchtop MALDI TOF mass spectrometer that has linear and reflector analyzer. It is used to analyze organic and inorganic compounds, protein identification, synthetic peptide validation, and polymer analysis.
Proper citation: Thermo Fisher: Applied Biosystems: ABI Voyager DE Pro MALDI-Mass Spectrometer (RRID:SCR_018432) Copy
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