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https://www.thermofisher.com/order/catalog/product/3730XL#/3730XL
96-capillary DNA analyzer that performs DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Has multiple automation features. Performs sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.
Proper citation: Thermo Fisher: Applied Biosystems: 3730xl Capillary Genetic Sequencer (RRID:SCR_018059) Copy
https://www.bio-rad.com/en-us/product/cfx96-touch-real-time-pcr-detection-system?ID=LJB1YU15
Real Time PCR detection system that combines optical technology with temperature control. Six channel, five colors and one FRET channel, real-time PCR instrument for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on integrated LCD touch screen, or use included CFX Maestro Software to design experiment and analyze results from connected computer.
Proper citation: Bio-Rad: CFX96 Real-Time PCR Detection System (RRID:SCR_018064) Copy
https://www.selectscience.net/products/zeiss-lsm-710-for-fluorescence-imaging/?prodID=84549#tab-2
Inverted microscope that enables confocal microscopy in cell and developmental biology.
Proper citation: Zeiss: LSM 710 Confocal Inverted Microscope (RRID:SCR_018063) Copy
https://www.biocompare.com/Product-Reviews/41153-LSM-510-META-From-Zeiss/
Versatile laser scanning confocal microscope system that is available in both upright or inverted format. Equipped with ICS-Optics. Selection of dry, water immersion and oil immersion objectives are available. ICS-Optics are infinity color corrected.
Proper citation: Zeiss: LSM 510 Confocal Microscope (RRID:SCR_018062) Copy
https://www.sonybiotechnology.com/us/instruments/sh800s-cell-sorter/
Benchtop cell sorter that sorts wide range of cell sizes for many applications using microfluidic sorting chips. Integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis. System software supports sorting into tubes and 96- and 384-well plates. Software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. For non-clinical research use only. For applications in which cross-contamination is concern, optional e-beam sterilized consumables can be used to replace sample line and sorting chip. Optional class A2 Level II biosafety cabinet provides protection for personnel and products. Biosafety cabinet meets international standards.Optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection.
Proper citation: Sony biotechnology: SH800S Cell Sorter (RRID:SCR_018066) Copy
https://www.ohio.edu/cas/genomics
Core provides equipment and services.Equipment available includes AB 3130xl Genetic Analyzer 16 capillary array,Nanodrop 1000 Agilent 2100 Bioanalyzer, Agilent 3100 Off Gel Fractionator, Sage Science Pippin Prep, Aria Mx Real Time PCR machine, Illumina MiSeq, Ion Torrent PGM. Research services include DNA sequencing, fragment and microsatellite analysis, AFLP, SNP screening RNA/DNA quantification, quality control, size distribution, RIN analysis, Protein fraction sizing and quanitification, QA/QC purified protein/antibodies, Low cell consumption two-color flow cytometry analysis, Isoelectric focusing prior to Mass Spec, DIGE analysis, or SDS-PAGE, Automated nucleic acid separation and size collection (90 bp to 1.5 kbp), Relative and Absolute qPCR quantification (SYBR, TaqMan probe), End-point PCR genotyping, fragment melt analysis, copy number determination. Provides Next-Generation Sequencing including total RNA, small RNA, exosome and rRNA-depleted RNAseq, SHAPE-seq, 5-end mapping, de novo genome sequencing and targeted DNA sequencing, ChIP-seq, Methyl-seq, Bioinformatic analysis of NGS data, Custom experimental design, optimization, and consulting. Educational services include Hands on training for any basic lab technique or service used at OUGF, Classroom and research lab lectures and informational seminars, Class and small group tours of facility.
Proper citation: Ohio University Genomics Core Facility (RRID:SCR_018268) Copy
THIS RESOURCE IS NO LONGER IN SERVICE. Documented on November 14,2023.Automated preparation system that isolates and prepares single cell templates for analysis. It can analyze mRNA and DNA sequencing, epigenetics or miRNA expression. It can extract, reverse transcribe, amplify, and analyze gene expression.
Proper citation: Fluidigm: C1 Single-Cell Automated Preparation System (RRID:SCR_018578) Copy
https://www.marshallscientific.com/Fisher-Scientific-PowerGen-125-Homogenizer-p/fs-pg125.htm
Homogenizer with speeds from 8,000 to 30,000 RPM for volumes of up to 100ml. Homogenizer for production of dispersions and emulsions, plant tissues, human tissues, animal tissues, resins, and pigments.
Proper citation: Thermo Fisher: n125 Homogenizer (RRID:SCR_018576) Copy
https://www.gilson.com/verity-271-hplc-system.html
HPLC liquid handler with midsize bed capacity and fraction collector. It has maximum flow rates up to 200 mL per min, injection loop volumes of 2 uL to 25 mL, and max sample mass of 15 grams per injection.
Proper citation: Gilson: GX-271 HPLC System (RRID:SCR_018579) Copy
https://www.ttuhsc.edu/pharmacy/research/documents/Amnis-Imagestream-Manual.pdf
Bench top multispectral imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. It combines per cell information with statistical significance of sample sizes of standard flow cytometry.
Proper citation: Luminex: Amnis ImageStreamX Mk II System (RRID:SCR_018589) Copy
https://www.illumina.com/documents/products/datasheets/datasheet_cbot.pdf
Genetic amplifier that uses amplification to create single molecule DNA templates and dispense reagents. It also controls reaction times, flow rates, and temperatures.
Proper citation: Illumina: cBot Genetic Amplifier (RRID:SCR_018580) Copy
https://www.kentscientific.com/products/coda-high-throughput-system/
CODA mouse rat tail-cuff system was designed to allow accurate blood pressure measurement in mice and rats. Blood pressure is measured in the tail of the mouse or rat using Volume Pressure Recording (VPR) sensor technology. Using the included software allows you to continuously view data in real-time.
Proper citation: Kent Scientific: Coda Blood Pressure System (RRID:SCR_018585) Copy
https://www.agilent.com/cs/library/brochures/5990-5596EN.pdf
Agilent 3100 OFFGEL Fractionator performs isoelectric focusing of proteins or peptides in immobilized pH gradient (IPG) gel strips.
Proper citation: Agilent: 3100 OFFGEL Fractionator (RRID:SCR_018433) Copy
https://mobile.labwrench.com/equipment/8044/applied-biosystems/voyager-de-pro
Benchtop MALDI TOF mass spectrometer that has linear and reflector analyzer. It is used to analyze organic and inorganic compounds, protein identification, synthetic peptide validation, and polymer analysis.
Proper citation: Thermo Fisher: Applied Biosystems: ABI Voyager DE Pro MALDI-Mass Spectrometer (RRID:SCR_018432) Copy
https://www.thelabworldgroup.com/mettler-toledo-pb303-precision-balance
Miligram balance that is used for weighing, tarring, percent weighing, and dynamic weighing for unstable samples. Has maximum capacity of 310 grams. Lab balance utilizes mono block weighing technology with permanent shock protection and built in overload protection as well as automatic calibration.
Proper citation: Mettler: PB303-S Milligram Balance (RRID:SCR_018592) Copy
Invitrogen benchtop automated cell counter. Can perform cell counts, viability calculations, hemocytometry, fluorescent protein expression, and measure fluorescence capabilities. There are also reusable slides, fluorescence capabilities, and brightfield fluorescence channels.
Proper citation: Thermo Fisher: Life Technologies: Countess Automated Cell Counter (RRID:SCR_018591) Copy
https://www.gmi-inc.com/product/packard-cobra-gamma-counter-series/
Gamma counter available in single, five, or ten detector configurations. Can count RIA tubes, 16x100mm and variety of smaller micro tubes using available adapter.This instrument has dot Matrix Printer, tube racks, and protocol ID tags.Single detector model is available in 2 inch or 3 inch crystal configurations for research grade capabilities.
Proper citation: Packard: Cobra 5000 Gamma Counter (RRID:SCR_018609) Copy
Dual channel fluorimeter for fluorescence measurements when calibrated with known concentration to measure compounds at low concentration with low assay volume.
Proper citation: Promega: TBS-380 Fluorimeter (RRID:SCR_018615) Copy
Sequencing and quantification platform for epigenetics, mutation gene expression analysis, and microbial identification and resistance typing. With its 96-well format, automatic base-calling function, and dedicated software solutions for methylation analysis and assay design.
Proper citation: Qiagen: PyroMark Q96 System (RRID:SCR_018617) Copy
Liquid handling system for production of complex liquid formulations produced from individual stock solutions of various chemical and liquid properties. With a CV of less than 2%.
Proper citation: Rigaku: Alchemist II Liquid Handler (RRID:SCR_018625) Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
