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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pREDIA
 
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RRID:Addgene_51625 I-SceI Saccharomyces cerevisiae Ampicillin PMID:24747889 Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Ampicillin 2026-08-15 01:16:15 0
pME-Gal80
 
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RRID:Addgene_51620 Gal80 Saccharomyces cerevisiae Kanamycin PMID:21905164 pME-Gal80 was cloned by PCR from TubP-GAL80 (Lee and Luo, 1999; obtained from Addgene, plasmid #17748) using primers 5'-ATGGACTACAACAAGAGATCTTCG-3' and 5'-TTATAAACTATAATGCGAGATATTGCT-3'. Vector Backbone:Gateway middle entry clone (pME); Vector Types:vertebrate expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:15 0
pREDTKI
 
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1+ mentions
RRID:Addgene_51628 I-SceI Saccharomyces cerevisiae Kanamycin PMID:24747889 Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Kanamycin 2026-08-15 01:16:15 3
M2371 his3::ADE2 Disruptor Converter
 
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RRID:Addgene_51671 ADE2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid was made by cutting pJJ215 with MscI and Nsi I and replacing the 570 bp MscI–Nsi I fragment with a 3.6 kb NotI (blunted with Klenow)–PstI fragment containing ADE2 from plasmid M1144. Plasmid M1144 contains a 3.6 kb BglII fragment (partial digest product) with ADE2 with BamHI linkers cloned into the BamHI site of Bluescript KS+. Vector Backbone:pJJ215; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
M3499 ura3::ADE2 Disruptor Converter
 
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1+ mentions
RRID:Addgene_51674 ADE2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid M3499 (ura3::ADE2 ) was made by cutting pJJ244 with StuI and EcoRV and replacing the 248 bp StuI–EcoRV fragment with a 3.6 kb BamHI blunted with Klenow) fragment containing ADE2 from plasmid M1144. Vector Backbone:pJJ244; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 1
D1433 his3::LYS2 Disruptor Converter
 
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RRID:Addgene_51676 LYS2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid was made by cutting YDp-H with BglII and replacing the 60 bp BglII fragment with a 5 kb BamHI fragment (partial digest product) containing LYS2 from plasmid YDp-K. Vector Backbone:YDp-H; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
M2660 ura3::LYS2 Disruptor Converter
 
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RRID:Addgene_51678 LYS2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid M2660 (ura3::LYS2 ) was made by cutting M581 with EcoRV and PstI and replacing the 208 bp EcoRV–PstI fragment with a 4.6 kb PvuII–PstI fragment containing LYS2 from plasmid YDp-K. Backbone Marker:Marsh et al., 1984; Vector Backbone:M581; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:20 0
M4755 KanMX::LEU2 Disruptor Converter
 
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RRID:Addgene_51685 LEU2 Saccharomyces cerevisiae Ampicillin PMID:12898713 M4756 (kanMX::LEU2)was made by digesting M4757 with BamHI and SphI and inserting a 2.0 kb BamHI–SphI fragment with LEU2 from plasmid pJJ250. Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
pCDNA3-ySet2
 
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RRID:Addgene_51698 yeast Set2 Saccharomyces cerevisiae Ampicillin PMID:20133523 Backbone Marker:commercial; Backbone Size:5400; Vector Backbone:pcDNA3.1 +; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
p413TEF-Sir2
 
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RRID:Addgene_51742 Sir2 Saccharomyces cerevisiae Ampicillin PMID:19390637 Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:20 0
pKF257
 
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RRID:Addgene_52533 I-SceI Saccharomyces cerevisiae Ampicillin PMID:24748663 Backbone Size:10877; Vector Backbone:pCASPER5; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:24 0
pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
 
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1+ mentions
RRID:Addgene_52889 Gal4 Saccharomyces cerevisiae Ampicillin PMID:25605786 Backbone Marker:David Strutt (Brittle et al., 2010); Vector Backbone:pAct5-FRT-stop-FRT-SV40polyA attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:31 3
pTK93_Lifeact-mCherry
 
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1+ mentions
RRID:Addgene_46357 Lifeact Saccharomyces cerevisiae Ampicillin PMID:23870127 Backbone Size:5000; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:31 9
pSNR52-sgTET
 
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RRID:Addgene_46923 sgRNA targeting endogenous TRE elements of pTET07 promoter Saccharomyces cerevisiae Ampicillin PMID:23849981 gRNA target sequence TATCAGTGATAGAGAAAAGT Backbone Size:5083; Vector Backbone:AH057; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:36 0
pJBEI-6410
 
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1+ mentions
RRID:Addgene_47049 Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene Saccharomyces cerevisiae Ampicillin PMID:23727191 The R364S mutation in the MK is not known to alter the function. The plasmid functions as described in the associated publication. Backbone Size:3500; Vector Backbone:pBbA5a; Vector Types:Bacterial Expression, Synthetic Biology, BglBrick; Bacterial Resistance:Ampicillin R364S mutation in MK 2026-08-15 01:15:37 2
pDR-meptrac-H194E
 
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RRID:Addgene_47764 MepTrac-H194E Saccharomyces cerevisiae Ampicillin PMID:23840931 Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin H194E 2026-08-15 01:15:44 0
pDR-meptrac
 
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RRID:Addgene_47768 meptrac Saccharomyces cerevisiae Ampicillin PMID:23840931 Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:45 0
pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
 
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1+ mentions
RRID:Addgene_53205 GAL1 promoter Saccharomyces cerevisiae Ampicillin GFP in Addgene plasmid #41614 was replaced with stable GFP variant (F64L, S65T, V163A). Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:16:32 1
pFA6a-GFP (F64L, S65T, V163A)-TRP1
 
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RRID:Addgene_53184 TRP1 Saccharomyces cerevisiae Ampicillin PMID:17942599 This plasmid is similar to Addgene plasmid #41597, except the GFP has been switched to a more stable variant. Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:16:28 0
pFA6a-GFP (F64L, S65T, V163A)-His3MX6
 
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RRID:Addgene_53185 HIs3MX6 Saccharomyces cerevisiae Ampicillin PMID:17942599 This plasmid is similar to Addgene plasmid #41598, except the GFP has been switched to a more stable variant. Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:16:28 0

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