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Species: E. coli
Genetic Insert: Colicin E1 and E1 immunity protein
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene.
Proper citation: RRID:Addgene_103063 Copy
Species: E. coli
Genetic Insert: colicin E1 immunity protein
Vector Backbone Description: Backbone Marker:Novagen EMD/Millipore; Backbone Size:5627; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The genes for colicin E1 with the R domain deletion and E1 immunity protein are transcribed from opposite strands of the vector. Colicin synthesis is under control of the vector inducible promoter; immunity protein synthesis is controlled by its own promoter.
Proper citation: RRID:Addgene_103065 Copy
Species: E. coli
Genetic Insert: sgGal4
Vector Backbone Description: Backbone Marker:Addgene #51024; Vector Backbone:pSLQ1651-sgRNA(F+E); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100549 Copy
Species: E. coli
Genetic Insert: RelA fragment from E.coli
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_100916 Copy
Species: E. coli
Genetic Insert: Ptrc promoter driving GalP transporter expression
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pAM1303; Vector Types:Cloning vector; Bacterial Resistance:Nourseothricin (clonNat)
References:
Comments:
Proper citation: RRID:Addgene_101843 Copy
Species: E. coli
Genetic Insert: Formaldehyde repressor protein (frmR)
Vector Backbone Description: Vector Backbone:pTR47m4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102436 Copy
Species: E. coli
Genetic Insert: yfaL
Vector Backbone Description: Backbone Size:3986; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene QC finds S92G, G99S, and K330R mutations in yfaL as well as a stop codon inserted before the His tag. The depositor notes these are not of functional concern
Proper citation: RRID:Addgene_110287 Copy
Species: E. coli
Genetic Insert: OmpW
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_112121 Copy
Species: E. coli
Genetic Insert: OmpT
Vector Backbone Description: Backbone Size:5636; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_112123 Copy
Species: E. coli
Genetic Insert: Lvl0 5'-2xTetApt-mVenus-PEST
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_115962 Copy
Species: E. coli
Genetic Insert: Lvl0 TetR SapI/BsaI Mut
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_115947 Copy
Species: E. coli
Genetic Insert: mcherry
Vector Backbone Description: Vector Backbone:pOSIP-KL; Vector Types:; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_116947 Copy
Species: E. coli
Genetic Insert: 2(KW)2-mCherry
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note: The mCherry insert contains a N9Q mutation. The depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_112738 Copy
Species: E. coli
Genetic Insert: 2HMG-eGFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_112737 Copy
Species: E. coli
Genetic Insert: neoPheS
Vector Backbone Description: Backbone Size:3470; Vector Backbone:pPB_LR5; Vector Types:Mammalian Expression, piggyBac transposon Gateway destination vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_112923 Copy
Species: E. coli
Genetic Insert: RFP
Vector Backbone Description: Vector Backbone:pBbE8K; Vector Types:Bacterial Expression, Cell-free; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_113109 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_113240 Copy
Species: E. coli
Genetic Insert: IHFa F13A
Vector Backbone Description: Backbone Size:3600; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_113244 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_113237 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_113236 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
