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Species: Coffee arabica
Genetic Insert: caffeine synthase 1 [Coffea arabica]
Vector Backbone Description: Vector Backbone:pSBBi-RP; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica caffeine synthase 1; the other side contains RFP and puromycin resistance genes.
Proper citation: RRID:Addgene_139162 Copy
Species: Camellia sinensis
Genetic Insert: Caffeine Synthase 1 [Camellia sinensis]
Vector Backbone Description: Vector Backbone:pSBBi-RP; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: SB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis caffeine synthase 1; the other side contains RFP and puromycin resistance genes.
Proper citation: RRID:Addgene_139161 Copy
Species: Camellia sinensis
Genetic Insert: Glutathione transferase + caffeine synthase 1 [Camellia sinensis]
Vector Backbone Description: Vector Backbone:pSBBi-RP; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: SB-transposon with constitutive bi-directional promoter. One side contains N-GST tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.
Proper citation: RRID:Addgene_139166 Copy
Species: Caenorhabditis elegans
Genetic Insert: mkate2 (no STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139046 Copy
Species: Caenorhabditis elegans
Genetic Insert: lov domain (includes STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139048 Copy
Species: Paullinia cupana
Genetic Insert: Predicted Paullinia cupana var sobilis Ancenstral Caffeine Synthase-2
Vector Backbone Description: Vector Backbone:pSBBi-RP; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana ancestral Caffeine Synthase 2 (PcAncCS2) the other side contains RFP and puromycin resistance genes.
Proper citation: RRID:Addgene_139170 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-54 3'UTR
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139053 Copy
Species: Caenorhabditis elegans
Genetic Insert: mkate2 (includes STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139050 Copy
Species: Coffee arabica
Genetic Insert: monomethylxanthine methyltransferase 1 [Coffea arabica]
Vector Backbone Description: Vector Backbone:pSBBi-RP; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase 1; the other side contains RFP and puromycin resistance genes.
Proper citation: RRID:Addgene_139172 Copy
Species: Homo sapiens
Genetic Insert: erythropoietin(EPO)
Vector Backbone Description: Vector Backbone:pLV-EF1alpha; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28602759
Proper citation: RRID:Addgene_139057 Copy
Genetic Insert: 3XHA-EGFP-PEX26
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32417403
Proper citation: RRID:Addgene_139054 Copy
Species: Homo sapiens
Genetic Insert: interleukin 3(IL3)
Vector Backbone Description: Vector Backbone:pLV-EF1alpha; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28602759
Proper citation: RRID:Addgene_139055 Copy
Species: Coffea arabica
Genetic Insert: CaMXMT
Vector Backbone Description: Vector Backbone:pSBTet-GB; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: SB-transposon with inducible SfiI cloning site containing dTomato-P2A-CaMXMT and constitutive expression of GFP and blasticidin resistance gene. Inducible gene expression can be stimulated by co-expression of TetOFF (e.g. tTA-advanced, TetRVP64).
Found mutation P131T on dTomato, depositor confirms this is not a concern
Proper citation: RRID:Addgene_139176 Copy
Species: Homo sapiens
Genetic Insert: colony stimulating factor 2 (CSF2)
Vector Backbone Description: Vector Backbone:pLV-EF1alpha; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28602759
Proper citation: RRID:Addgene_139058 Copy
Genetic Insert: 3XMyc-EGFP-PEX26
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32417403
Proper citation: RRID:Addgene_139059 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Modified pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9608511
Comments: pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector.
pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site.
For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html
Proper citation: RRID:Addgene_13918 Copy
Species: Homo sapiens
Genetic Insert: KRAS 3'UTR
Vector Backbone Description: Backbone Marker:promega; Backbone Size:0; Vector Backbone:pGL3 promoter; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15766527
Proper citation: RRID:Addgene_13919 Copy
Species: Mus musculus
Genetic Insert: PrP
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pCB6+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11742063
Comments: Full length mouse PrP with 3F4 epitag was cloned into HindIII and BamHI sites of mammalian cell expression vector pCB6+.
Proper citation: RRID:Addgene_13917 Copy
Vector Backbone Description: Backbone Size:4643; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: Empty SB-transposon with STAT3 inducible promoter and constitutive bleomycin resistance.
Proper citation: RRID:Addgene_139184 Copy
Species: Other
Genetic Insert: ABE RA6.3
Vector Backbone Description: Vector Backbone:lenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31740768
Proper citation: RRID:Addgene_139104 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
