Plasmids   Select Another Resource Report Type

http://www.addgene.org/41892

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pSR47S; Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB; Bacterial Resistance:Chloramphenicol
References:
Comments: Plasmid pSR47S Cm was constructed by removing the KmR region from pSR47S by PCR amplification of the residual plasmid, then cloning in the CmR gene. The CmR gene was amplified from a pMMB207 plasmid by PCR, with restriction sites that allowed cloning into pSR47S that lacked the KmR region.

Proper citation: RRID:Addgene_41892 Copy   

•   Source: Addgene

http://www.addgene.org/28039

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7484; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: pTGV-Express contains PBAD and also includes the Shine-Dalgarno prokaryotic translation-initiation sequence for expression of heterologous genes in E. coli. A single nucleotide deletion was detected in Addgene's quality control sequence compared to the depositing scientist's sequence at bp# 3277. The depositing scientist says this deletion has no impact on the function of the plasmid.

Proper citation: RRID:Addgene_28039 Copy   

•   Source: Addgene

http://www.addgene.org/28033

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7240; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Note that Addgene's sequencing results found a 1 nucleotide mismatch at bp# 238 and 1 nucleotide deletions at bp# 2970 & 3016 when compared with the sequence provided by the depositing laboratory. These differences are not known to affect plasmid function.

Proper citation: RRID:Addgene_28033 Copy   

•   Source: Addgene

http://www.addgene.org/21842

Species: L. pneumophila
Genetic Insert: fabI
Vector Backbone Description: Backbone Size:9869; Vector Backbone:pXDC61; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:

Proper citation: RRID:Addgene_21842 Copy   

•   Source: Addgene

http://www.addgene.org/21867

Species: Mesoplasma florum
Genetic Insert: Lon
Vector Backbone Description: Backbone Marker:Beckwith Lab; Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: A detailed protocol for purification of mf-lon can be found in the associated paper.

Proper citation: RRID:Addgene_21867 Copy   

•   Source: Addgene

http://www.addgene.org/22819

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3586; Vector Backbone:pBTB-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.

Proper citation: RRID:Addgene_22819 Copy   

•   Source: Addgene

http://www.addgene.org/22869

Species: Synechocystis/E.coli fusion gene
Genetic Insert: pCph8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:2021; Vector Backbone:pPROTet; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: This plasmid was also used in the 2009 Cell paper: "A synthetic genetic edge detection program." PMID:19563759

Proper citation: RRID:Addgene_22869 Copy   

•   Source: Addgene

http://www.addgene.org/22826

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4831; Vector Backbone:pBMTB-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.

Proper citation: RRID:Addgene_22826 Copy   

•   Source: Addgene

http://www.addgene.org/22838

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3649; Vector Backbone:pBMT-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:

Proper citation: RRID:Addgene_22838 Copy   

•   Source: Addgene

http://www.addgene.org/23011

Species: Mus musculus
Genetic Insert: DHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pBAD/HisA & pBThybrid; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:

Proper citation: RRID:Addgene_23011 Copy   

•   Source: Addgene

http://www.addgene.org/34617

Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:2070; Vector Backbone:pPROLar.A122; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: MG1655 for expression

Proper citation: RRID:Addgene_34617 Copy   

•   Source: Addgene

http://www.addgene.org/34625

Species: Sperm Whale: Physeter macrocephalus
Genetic Insert: Myoglobin-127TAG
Vector Backbone Description: Vector Backbone:pSepT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.

Proper citation: RRID:Addgene_34625 Copy   

•   Source: Addgene

http://www.addgene.org/34879

Species:
Genetic Insert: SB100X transposase
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.

Proper citation: RRID:Addgene_34879 Copy   

•   Source: Addgene

http://www.addgene.org/35152

Species: Saccharomyces cerevisiae
Genetic Insert: AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
Vector Backbone Description: Vector Backbone:pBbA5c; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Please note that the R364 in Mevalonate Kinase (MK) may be susceptible to mutations. Although the original plasmid encoded R364C, Addgene's stock contains R364S. This mutation is not expected to impact overall function, however the exact effects are unknown.

Proper citation: RRID:Addgene_35152 Copy   

•   Source: Addgene

http://www.addgene.org/37980

Species:
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Strain description: CsrA system knockout Chromosomal changes: ΔCsrA, ΔCsrB, ΔCsrC, ΔglgCAP, ΔpgaABCD, +LacIq at galK

Proper citation: RRID:Addgene_37980 Copy   

•   Source: Addgene

http://www.addgene.org/37976

Species:
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Host strain for pHL32B

Proper citation: RRID:Addgene_37976 Copy   

•   Source: Addgene

http://www.addgene.org/37977

Species:
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Host strain for pHL32B Strain description: ΔDam Chromosomal changes: ΔDam

Proper citation: RRID:Addgene_37977 Copy   

•   Source: Addgene

http://www.addgene.org/37979

Species:
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Host strain for pHL32B Strain description: Δ(Dam+OxyR) Chromosomal changes: Δ(Dam+OxyR)

Proper citation: RRID:Addgene_37979 Copy   

•   Source: Addgene

http://www.addgene.org/38207

Species:
Genetic Insert: Bxb1-Excisionase
Vector Backbone Description: Backbone Size:2800; Vector Backbone:J64100; Vector Types:Synthetic Biology, BioBrick cloning vector; Bacterial Resistance:Chloramphenicol
References:
Comments: In the reset circuit, PBAD controls expression of a polycistron encoding excisionase with a strong RBS followed by Bxb1 integrase with a GTG start codon to decrease its translation rate and increase the excisionase to integrase ratio. This construct was cloned in a plasmid with a regulated ColE1 origin of replication (J64100, 50-70 copies, chloramphenicol resistance).

Proper citation: RRID:Addgene_38207 Copy   

•   Source: Addgene

http://www.addgene.org/39192

Species: Homo sapiens
Genetic Insert: AASDHPPT
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pCOEX-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: http://www.thesgc.org/structures/2BYD/

Proper citation: RRID:Addgene_39192 Copy   

•   Source: Addgene