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Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227770 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sgd1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found T307A, S332F, and V832F in the Sgd1 translation compared to the best-match NCBI reference sequence (GAA25198.1).
Proper citation: RRID:Addgene_100000 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS511b
Vector Backbone Description: Vector Backbone:p426; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27899650
Proper citation: RRID:Addgene_87387 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mitochondrial FAD-linked sulfhydryl oxidase
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176405 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found S1073N, and an extra 16 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100003 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found S1073N, and an extra 30 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100002 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast genes
Vector Backbone Description: Backbone Marker:Addgene 35452; Vector Backbone:pRS41x; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33757429
Proper citation: RRID:Addgene_161769 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Size:3462; Vector Backbone:pQE30 (QIAGEN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:12761287
Proper citation: RRID:Addgene_16232 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDC3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11577116
Proper citation: RRID:Addgene_16252 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: Impaired Edc3 binding sites (first HLM, and 5 additional HLMs in C-terminal domain).
5' sequencing primers:
Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163582 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: C-terminal domain (327-970) containing 5 impaired Edc3 binding sites (5 additional HLMs in C-terminal domain); N-terminal domain (1-299) including first HLM is removed.
5' sequencing primers:
Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163581 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: 5' sequencing primers: Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163585 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: Impaired Edc3 binding site (first HLM); impaired RNA cap recognition site (W50, D54).
5' sequencing primers:
Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163580 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: 5' sequencing primers: Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163574 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:5827; Vector Backbone:pRP1903; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32553117
Comments: Lacking C-terminal domain.
5' sequencing primers:
Dcp2-seq1b:CGAATTTTACGTCTGAGGAAAG. Dcp2-seq2b: gacatagattgttgcattag. Dcp2-seq3b: cttcagcatttgaaagagca Dcp2-seq4b:gcaaaacaataatgatgaaa Dcp2-seq5b: gagaacaatttccaagacgg. Dcp2-seq7:tgaaacgcaacgacgctaca Dcp2-seq8b: GATACCCAGATCATATGAAACG
Proper citation: RRID:Addgene_163577 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MatA
Vector Backbone Description: Vector Backbone:pCR™-Blunt II-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29150593
Proper citation: RRID:Addgene_169457 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA for MATα
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29150593
Proper citation: RRID:Addgene_169454 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ecm2 1-143
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5000; Vector Backbone:pRS416; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33547186
Proper citation: RRID:Addgene_169923 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ecm2 1-325
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5000; Vector Backbone:pRS416; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33547186
Proper citation: RRID:Addgene_169926 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 U57A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33547186
Comments: Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone.
Proper citation: RRID:Addgene_169927 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
