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Vector Backbone Description: Backbone Marker:Cambia; Vector Backbone:pCAMBIA1300; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:26644504
Comments: See genbank file for best annotation of features.
Proper citation: RRID:Addgene_71275 Copy
Vector Backbone Description: Backbone Marker:Thermo; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:30379827
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
Proper citation: RRID:Addgene_71534 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9000; Vector Backbone:pBI101; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:21343429
Comments: Aoyama, T. and Chua, N.-H. (1997) A glucocorticoid-mediated
transcriptional induction system in transgenic plants. Plant J.
11, 605–612
Proper citation: RRID:Addgene_71745 Copy
Vector Backbone Description: Backbone Size:7450; Vector Backbone:pDEST1K18ms; Vector Types:sucrose counter-selection allelic exchange vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:34032522
Proper citation: RRID:Addgene_72646 Copy
Species: Pseudomonas putida strain CBB5
Genetic Insert: ndmA
Vector Backbone Description: Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23654268
Comments: The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3.
Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100).
N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See:
Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049.
For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC).
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_65220 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:27084942
Comments: Please note that parental strain (MC4100) is streptomycin-resistant.
Proper citation: RRID:Addgene_69771 Copy
Species: Synthetic
Genetic Insert: Splitposon w/ KanR, LacI & PTac
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59947 Copy
Species: Oryza sativa
Genetic Insert: OsMIR390-B/c
Vector Backbone Description: Backbone Size:9620; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25809382
Proper citation: RRID:Addgene_61466 Copy
Genetic Insert: C terminal VSV tag
Vector Backbone Description: Backbone Size:2852; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26181 Copy
Genetic Insert: C terminal HA tag
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26174 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Med21 aa1-132 for N-terminal tagging
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26183 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51778 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51775 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51774 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54382 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54381 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54388 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54386 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54384 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54393 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
