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Species: Saccharomyces cerevisiae
Genetic Insert: Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS511b
Vector Backbone Description: Vector Backbone:p426; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27899650
Proper citation: RRID:Addgene_87387 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mitochondrial FAD-linked sulfhydryl oxidase
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pACYC184-TU2A-RFP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.31.458447v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176405 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sgd1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found T307A, S332F, and V832F in the Sgd1 translation compared to the best-match NCBI reference sequence (GAA25198.1).
Proper citation: RRID:Addgene_100000 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EL222 codon-optimized for budding yeast
Vector Backbone Description: Vector Backbone:EZL105; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.
Proper citation: RRID:Addgene_227770 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Csy4
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5522; Vector Backbone:p413 TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161506
Proper citation: RRID:Addgene_103139 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pDTU-113; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161506
Proper citation: RRID:Addgene_103140 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNAs
Vector Backbone Description: Vector Backbone:dCas9v2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161506
Proper citation: RRID:Addgene_103141 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pAdh1-Msn2-mCherry
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28035051
Proper citation: RRID:Addgene_103844 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGal1-Ras2(KAN)
Vector Backbone Description: Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28035051
Proper citation: RRID:Addgene_103846 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FBP1
Vector Backbone Description: Backbone Size:8017; Vector Backbone:pJO629; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28126757
Comments: Replace the Yap5 gene of pJO629 to FBP1 gene.
Proper citation: RRID:Addgene_104343 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUP1
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18708352
Proper citation: RRID:Addgene_104341 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSN6
Vector Backbone Description: Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18708352
Comments: Addgene NGS result found SSN6 with P40Q variant.
Proper citation: RRID:Addgene_104340 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for ATP sulfurylase
Proper citation: RRID:Addgene_107436 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for homocysteine methyltransferase
Proper citation: RRID:Addgene_107438 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET14
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for adenylylsulfate kinase
Proper citation: RRID:Addgene_107441 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for the alpha subunit of sulfite reductase
Proper citation: RRID:Addgene_107440 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5577; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5593; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107923 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5775; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107922 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5280; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107931 Copy
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