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Species: Renilla reniformis
Genetic Insert: Renilla
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2343; Vector Backbone:pUPD3; Vector Types:Luciferase, Synthetic Biology, GBPart; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: Insert can be released with BsaI
Proper citation: RRID:Addgene_118059 Copy
Species: Synthetic
Genetic Insert: ELuc
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2343; Vector Backbone:pUPD3; Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: Insert can be released with BsaI
Proper citation: RRID:Addgene_118056 Copy
Species: Synthetic
Genetic Insert: minimal TATA-box promoter with low basal activity
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2343; Vector Backbone:pUPD3; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: Insert can be released with BsaI
Proper citation: RRID:Addgene_118049 Copy
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2171; Vector Backbone:ColE1 vector Omega1; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology, Assembly of GB2.0 transcriptional units; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: This is a BmsBI GB2.0 Omega entry vector. Any assembled unit(s) can be released with BsaI for further cloning into Alfa level vectors.
Proper citation: RRID:Addgene_118046 Copy
Species: Homo sapiens
Genetic Insert: 2 copies of the p53 DNA binding motif
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2343; Vector Backbone:pUPD; Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: Insert can be released with BsaI
Proper citation: RRID:Addgene_118053 Copy
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2804; Vector Backbone:pUPD; Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: This is a GB2.0 domesticator vector. GBParts can be assembled in a BsmBI-ligation reaction. Any assembled Part can be released with BsaI for further cloning into Alpha level vectors.
Proper citation: RRID:Addgene_118043 Copy
Species: Synthetic
Genetic Insert: RBS, amilGFP
Vector Backbone Description: Backbone Marker:iGEM Registry; Vector Backbone:BioBrick pSB1C3 vector; Vector Types:Synthetic Biology, Escherichia coli; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29760772
Proper citation: RRID:Addgene_118006 Copy
Genetic Insert: ZB-linker-split C-terminal T7 RNAP expression plasmid
Vector Backbone Description: Vector Backbone:custom; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28192413
Proper citation: RRID:Addgene_118086 Copy
Species: Other
Genetic Insert: B. subtilis deltaTn7
Vector Backbone Description: Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30617347
Comments:
IMPORT NOTES: Special character found in field(Gene or insert Name)
Proper citation: RRID:Addgene_119266 Copy
Species: Other
Genetic Insert: B. subtilis attTn7
Vector Backbone Description: Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119264 Copy
Genetic Insert: Cas9 from S. pyogenes
Vector Backbone Description: Vector Backbone:p15A, Chloramphenicol resistance; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32995532
Proper citation: RRID:Addgene_119162 Copy
Species: Cochliobolus heterostrophus (Pgpda), Herpes simplex virus (HSVtk), Neurospora crassa (Ttub)
Genetic Insert: Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29680684
Proper citation: RRID:Addgene_119710 Copy
Species: Penicillium chrysogenum
Genetic Insert: Terminator paf
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29680684
Proper citation: RRID:Addgene_119714 Copy
Species: Pseudoalteromonas translucida
Genetic Insert: Mini-Tn, GFP cassette
Vector Backbone Description: Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34478496
Proper citation: RRID:Addgene_175578 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XII-5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176177 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dropout cassette containing toxic genes ccdB and TPK2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015).
Proper citation: RRID:Addgene_176179 Copy
Species: Synthetic
Genetic Insert: 41 bp non-coding DNA spacer from pYTK048
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015).
Proper citation: RRID:Addgene_176178 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site XI-3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176170 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site XI-5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176172 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XII-2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176175 Copy
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