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http://www.addgene.org/111003

Species: Synthetic
Genetic Insert: secreted ookinete protein (PSOP1)
Vector Backbone Description: Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_111003 Copy   

•   Source: Addgene

http://www.addgene.org/111004

Species: Synthetic
Genetic Insert: CPN60
Vector Backbone Description: Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_111004 Copy   

•   Source: Addgene

http://www.addgene.org/111001

Species: Synthetic
Genetic Insert: aminopeptidase P (APP)
Vector Backbone Description: Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_111001 Copy   

•   Source: Addgene

http://www.addgene.org/110953

Species: Homo sapiens
Genetic Insert: ZNHIT3
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_110953 Copy   

•   Source: Addgene

http://www.addgene.org/110950

Species: Homo sapiens
Genetic Insert: NUFIP1
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_110950 Copy   

•   Source: Addgene

http://www.addgene.org/110951

Species: Homo sapiens
Genetic Insert: NUFIP1 W40A
Vector Backbone Description: Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_110951 Copy   

•   Source: Addgene

http://www.addgene.org/110959

Species: Synthetic
Genetic Insert: conserved Plasmodium protein, unknown function
Vector Backbone Description: Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_110959 Copy   

•   Source: Addgene

http://www.addgene.org/1110

Species: Saccharomyces cerevisiae
Genetic Insert: HSP82
Vector Backbone Description: Backbone Marker:ATCC (#77189); Backbone Size:4453; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_1110 Copy   

•   Source: Addgene

http://www.addgene.org/11100

Species: Mus musculus
Genetic Insert: NFAT1
Vector Backbone Description: Backbone Marker:Genetics Institute; Backbone Size:0; Vector Backbone:GFP-RV-DV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_11100 Copy   

•   Source: Addgene

http://www.addgene.org/110941

Species: Homo sapiens
Genetic Insert: shOGDH
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Recombination can be minimized by using NEB Stable E. coli strain grown at 30C to amplify the plasmid DNA. Even so, low recombination rate may still happen and when you eventually amplify the DNA you may need to screen through several colonies and perform diagnostic digests to identify the correct ones.

Proper citation: RRID:Addgene_110941 Copy   

•   Source: Addgene

http://www.addgene.org/110940

Species:
Genetic Insert: shRFP
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Recombination can be minimized by using NEB Stable E. coli strain grown at 30C to amplify the plasmid DNA. Even so, low recombination rate may still happen and when you eventually amplify the DNA you may need to screen through several colonies and perform diagnostic digests to identify the correct ones.

Proper citation: RRID:Addgene_110940 Copy   

•   Source: Addgene

http://www.addgene.org/110945

Species: Homo sapiens
Genetic Insert: E3 ubiquitin ligase parkin
Vector Backbone Description: Backbone Size:4698; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_110945 Copy   

•   Source: Addgene

http://www.addgene.org/110946

Species: Homo sapiens
Genetic Insert: Dynamin-1-like protein
Vector Backbone Description: Backbone Marker:Gia Voeltz lab; Backbone Size:3833; Vector Backbone:mCh-Drp1 (Plasmid #49152); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_110946 Copy   

•   Source: Addgene

http://www.addgene.org/110949

Species: Synthetic
Genetic Insert: ccd B cassette
Vector Backbone Description: Backbone Marker:Yingfeng An; Vector Backbone:pANY1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: During molecular cloning, DNA of interest can be inserted into pANY1 using both MCS-1 and MCS-2, with deletion of the ccdB cassette. Thus the recombinant plasmid can be transformed into commonly used E. coli strains (e.g., JM109, BL21, DH5a). Because the original plasmid, pANY1, cannot be transformed into these E. coli strains, false positives can be eliminated.

Proper citation: RRID:Addgene_110949 Copy   

•   Source: Addgene

http://www.addgene.org/110948

Species: Homo sapiens
Genetic Insert: Lysosomal-associated membrane protein 1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pAc5.1/V5-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_110948 Copy   

•   Source: Addgene

http://www.addgene.org/110930

Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Backbone Size:2632; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Unique multi-cloning sites: Nhe I, Sbf I, Kpn I/ Age I/ Xho I, Bgl II This vector is the same as KG#59, except the Sal I and Acc I sites of the multi-cloning site have been replaced with a Sbf I site. Synthesized 2 complementary oligos containing Nhe I and Age I sticky ends and Sbf I and Kpn I sites in between. Hybridization of the 2 oligos to each other will produce Nhe I and Age I sticky ends. After hybridization, this synthetic insert (which is non-phosphorylated on its 5' termini) was cloned into Nhe I/ Age I cut KG#59. Transformed into XL1-Blue electrocompetent cells. Miniprepped 6 clones and tested for the Sbf I site by cutting with Sbf I. Chose one clone that has the Sbf I site with correct band size (and only a single band) and made the glycerol stock. Features of the expression construct: This expression construct has a promoter (rab-3) that will drive strong and specific expression (of whatever gene is inserted in the MCS) in the entire nervous system of juvenile and adult animals. The boundaries of the rab-3 promoter sequence were obtained by examining pRabGFPrim3' from Mike Nonet's lab web site and comparing that to the genome sequence. Other features of the expression vector include a "decoy" 5' to the promoter to reduce non-specific expression in the gut (see Fire Lab 1995 Kit documentation for description), the unc-54 3' end including the poly A addition signal, and 3 introns (1 in 5' UTR, 1 just upstream of the unc-54 3' end, and 1 inserted in the unc-54 3' end). These introns help provide more uniform and robust expression, especially if you are expressing a cDNA with no introns as opposed to a gene with introns. Note: this vector can be converted to a vector that fuses GFP, YFP, or CFP onto the C-terminus of the protein as follows: Remove the ~1000 bp (Kpn I or Age I) + (Spe I or BsiW I [expensive, 55 C cutter with 50% activity at 37 C; heat inactivate 80 C] or Apa I) fragment containing the unc-54 3’ control region from this plasmid and replace it with the ~1800 bp like-digested fragment containing 3-intron S65C GFP (see pPD94.81 in Fire Lab plasmids), YFP (see pPD136.64 in Fire Lab plasmids) or CFP (see pPD136.61 in Fire Lab plasmids) + the unc-54 3’ UTR and control region. Note if Spe I is used to cut these Fire Lab vectors, you get 3 bands: 1100, 1800, and 4000 (total size 6.9 Kb). The 1800 bp band is the XFP-containing band. For C-terminal fusions, remember to leave the stop codon off of the upstream protein. For the above-mentioned Fire Lab vectors, the reading frame for the downstream XFP relative to the Kpn I and Age I sites is as follows (triplets are the reading frame codons): Kpn I G GTA CCG GT Age I Alternatively, use Gibson Assembly/ NEBuilder to insert any genetically encoded fluorescent protein at the N- or C-terminus of your protein.

Proper citation: RRID:Addgene_110930 Copy   

•   Source: Addgene

http://www.addgene.org/110931

Species: Caenorhabditis elegans
Genetic Insert: unc-17 promoter
Vector Backbone Description: Backbone Size:2632; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Unique multi-cloning sites: Nhe I, Sal I, Kpn I/ Age I/ Eco RV, Bgl II Used Apa I/ Msc I to cut out the ~1900 bp GFP/ 3' control region from RM#349p, leaving the 5900 bp vector fragment containing the unc-17 full promoter + remaining vector sequences. To this vector fragment, we ligated the 1079 bp Apa I/ Msc I fragment cut from pPD96.52 (C. elegans body wall muscle expression vector). Picked 6 colonies for minipreps. Chose 1 clone with correct size insert and made glycerol stock. Features of the expression construct: This is a C. elegans expression vector with the unc-17 3.2 Kb promoter + a large MCS for cloning in cDNAs. Includes cha-1/unc-17 upstream region from Sna-1 (=Bst1107 I = GTA/TAC) down to first half of the 66 bp first exon (which is common to unc-17 and cha-1 transcripts but is not translated). Includes beta site so that expression occurs in most cholinergic cells. Expression still missing from VC's and some cholinergic head and tail neurons. In this construct the GFP/ 3' control region of RM#349p is replaced with the MCS II + 3' control region of pPD96.52. This includes the unc-54 3' end including the poly A addition signal, and 3 introns (1 in 5' UTR, 1 just upstream of the unc-54 3' end, and 1 inserted in the unc-54 3' end). These introns help provide more uniform and robust expression, especially if you are expressing a cDNA with no introns as opposed to a gene with introns. Note: this vector can be converted to a vector that fuses GFP, YFP, or CFP onto the C-terminus of the protein as follows: Remove the ~1000 bp (Kpn I or Age I) + (Spe I or BsiW I [expensive, 55 C cutter with 50% activity at 37 C; heat inactivate 80 C] or Apa I) fragment containing the unc-54 3’ control region from this plasmid and replace it with the ~1800 bp like-digested fragment containing 3-intron S65C GFP (see pPD94.81 in Fire Lab plasmids), YFP (see pPD136.64 in Fire Lab plasmids) or CFP (see pPD136.61 in Fire Lab plasmids) + the unc-54 3’ UTR and control region. Note if Spe I is used to cut these Fire Lab vectors, you get 3 bands: 1100, 1800, and 4000 (total size 6.9 Kb). The 1800 bp band is the XFP-containing band. For C-terminal fusions, remember to leave the stop codon off of the upstream protein. For the above-mentioned Fire Lab vectors, the reading frame for the downstream XFP relative to the Kpn I and Age I sites is as follows (triplets are the reading frame codons): Kpn I G GTA CCG GT Age I Alternatively, use Gibson Assembly/ NEBuilder to insert any genetically encoded fluorescent protein at the N- or C-terminus of your protein.

Proper citation: RRID:Addgene_110931 Copy   

•   Source: Addgene

http://www.addgene.org/110932

Species: Caenorhabditis elegans
Genetic Insert: unc-17 promoter
Vector Backbone Description: Backbone Size:2632; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Unique multi-cloning sites: Nhe I, Sbf I, Kpn I/ Age I/ Eco RV, Bgl II Note: this vector is the same as KG#94, except a Sbf I site replaces the Sal I site. Synthesized 2 complementary oligos containing Nhe I and Age I sticky ends and Sbf I and Kpn I sites in between. Hybridization of the 2 oligos to each other should produce Nhe I and Age I sticky ends. After hybridization, this synthetic insert (which is non-phosphorylated on its 5' termini) was cloned into Nhe I/ Age I cut KG#94. Transformed into XL1-Blue electrocompetent cells. Miniprepped 4 clones and tested for the Sbf I site by cutting with Sbf I. Chose one clone that has the Sbf I site with correct band size (and only a single band) and made a glycerol stock. Features of the expression construct: This is a C. elegans expression vector with the unc-17 3.2 Kb promoter + a large MCS for cloning in cDNAs. Includes cha-1/unc-17 upstream region from Sna-1 (=Bst1107 I = GTA/TAC) down to first half of the 66 bp first exon (which is common to unc-17 and cha-1 transcripts but is not translated). Includes beta site so that expression occurs in most cholinergic cells. Expression still missing from VC's and some cholinergic head and tail neurons. In this construct the GFP/ 3' control region of RM#349p is replaced with the MCS II + 3' control region of pPD96.52. This includes the unc-54 3' end including the poly A addition signal, and 3 introns (1 in 5' UTR, 1 just upstream of the unc-54 3' end, and 1 inserted in the unc-54 3' end). These introns help provide more uniform and robust expression, especially if you are expressing a cDNA with no introns as opposed to a gene with introns. Note: this vector can be converted to a vector that fuses GFP, YFP, or CFP onto the C-terminus of the protein as follows: Remove the ~1000 bp (Kpn I or Age I) + (Spe I or BsiW I [expensive, 55 C cutter with 50% activity at 37 C; heat inactivate 80 C] or Apa I) fragment containing the unc-54 3’ control region from this plasmid and replace it with the ~1800 bp like-digested fragment containing 3-intron S65C GFP (see pPD94.81 in Fire Lab plasmids), YFP (see pPD136.64 in Fire Lab plasmids) or CFP (see pPD136.61 in Fire Lab plasmids) + the unc-54 3’ UTR and control region. Note if Spe I is used to cut these Fire Lab vectors, you get 3 bands: 1100, 1800, and 4000 (total size 6.9 Kb). The 1800 bp band is the XFP-containing band. For C-terminal fusions, remember to leave the stop codon off of the upstream protein. For the above-mentioned Fire Lab vectors, the reading frame for the downstream XFP relative to the Kpn I and Age I sites is as follows (triplets are the reading frame codons): Kpn I G GTA CCG GT Age I Alternatively, use Gibson Assembly/ NEBuilder to insert any genetically encoded fluorescent protein at the N- or C-terminus of your protein.

Proper citation: RRID:Addgene_110932 Copy   

•   Source: Addgene

http://www.addgene.org/110938

Species: Homo sapiens
Genetic Insert: OGDH wobble cDNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9387; Vector Backbone:pLenti6.3/V5-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_110938 Copy   

•   Source: Addgene

http://www.addgene.org/110939

Species:
Genetic Insert: shGFP
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Recombination can be minimized by using NEB Stable E. coli strain grown at 30C to amplify the plasmid DNA. Even so, low recombination rate may still happen and when you eventually amplify the DNA you may need to screen through several colonies and perform diagnostic digests to identify the correct ones.

Proper citation: RRID:Addgene_110939 Copy   

•   Source: Addgene