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Species: Homo sapiens
Genetic Insert: Human H2B.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:Pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42630 Copy
Species: Homo sapiens
Genetic Insert: Human H3.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:Pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42631 Copy
Species: Homo sapiens
Genetic Insert: Human H2AZ.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:Pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42629 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: N-myristoyl tranferase
Vector Backbone Description: Backbone Marker:Novagen/EMD Millipore; Backbone Size:3600; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Used for production of myristylated HIV-1 Gag in E. coli.
Proper citation: RRID:Addgene_42578 Copy
Species: Mus musculus
Genetic Insert: LAP-neurexin-1beta
Vector Backbone Description: Backbone Size:3897; Vector Backbone:pNICE; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42575 Copy
Species: HPV16
Genetic Insert: HPV16 E6
Vector Backbone Description: Backbone Size:8100; Vector Backbone:MSCV-IP N-HAonly; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42603 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:NOVAGEN; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42606 Copy
Species:
Genetic Insert: LacZ
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there are a few minor sequence discrepancies between the provided depositor's sequence and Addgene quality control sequence, most notably the 6xHis tag is missing.
These changes should not affect function and this plasmid can still be used as control.
Proper citation: RRID:Addgene_42560 Copy
Species: Homo sapiens
Genetic Insert: TBX5
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLX303; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42563 Copy
Species: Rattus norvegicus
Genetic Insert: ankyrin G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42566 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-EMP2-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32290; Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TCCCACACAGCACAAGAT
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42710 Copy
Species:
Genetic Insert: shLacZ
Vector Backbone Description: Backbone Marker:TRC; Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42559 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-EMP2-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TCAGGCAGTGCAGGCCAC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42709 Copy
Species: Homo sapiens
Genetic Insert: EGFR ECD (1-644)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42666 Copy
Species: Homo sapiens
Genetic Insert: EGFR ICD (645-1186)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42667 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there is an additional mutation in EZH2 ORF, Q3R. The mutation does not affect plasmid function.
Proper citation: RRID:Addgene_42664 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42665 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-oxtr-right
Vector Backbone Description: Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TGGTGGCGTTTCTGGATG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42794 Copy
Species: Danio rerio
Genetic Insert: ZebrafishCommunity-oxtrl-left
Vector Backbone Description: Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
References:
Comments: Target binding site: TGATCCTCTGGCCCATTA
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb.
Proper citation: RRID:Addgene_42795 Copy
Species:
Genetic Insert: attL1::egfp (870 bp)::attL2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR 221; Vector Types:Multi-site Gateway donor plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42930 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
