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Species: Homo sapiens
Genetic Insert: PSMA1
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_217893 Copy
Species: Homo sapiens
Genetic Insert: PSMB1
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_217895 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pHBJT021; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: pHBJT021 containing IPTG-inducible mCherry
Proper citation: RRID:Addgene_225179 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3900; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: Low copy plasmid (pSC101 ori and repA), pUC19 MCS, confers gentamycin resistance
Proper citation: RRID:Addgene_225172 Copy
Species:
Genetic Insert: Cas13a(Lse)
Vector Backbone Description: Vector Backbone:pUC18-mini-Tn7T-LAC; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_194341 Copy
Species:
Genetic Insert: crRNA(Lse)
Vector Backbone Description: Vector Backbone:pHERD30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_194476 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3663; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: Low copy plasmid (pSC101 ori and repA), pUC19 MCS with FLAG epitope integrated at SmaI site, confers gentamycin resistance
Proper citation: RRID:Addgene_225162 Copy
Species: Synthetic
Genetic Insert: cdGreen2.1
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:4300; Vector Backbone:pAK206; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_221150 Copy
Species:
Genetic Insert: pBAD_dCas9sth1
Vector Backbone Description: Vector Backbone:pAL5000/pMB1 shuttle vector; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_119880 Copy
Species: Pseudomonas putida DOT-T1E
Genetic Insert: single-stranded DNA recombineering
Vector Backbone Description: Backbone Marker:SEVA collection; Backbone Size:5089; Vector Backbone:pSEVA624; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_199241 Copy
Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: Replicating ORBIT V2 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. V2 plasmid does not have mutL, which lowers background mutation rate. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205293 Copy
Species:
Genetic Insert: TevSpCas9, sgRNA cassette, oriT, CenArsHis
Vector Backbone Description: Backbone Size:52707; Vector Backbone:pTA-Mob; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_202796 Copy
Species: Homo sapiens
Genetic Insert: SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV SpCas9; Vector Types:Mammalian Expression, CRISPR, Recombinant baculovirus production; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_206267 Copy
Species: Other
Genetic Insert: H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Recombinant baculovirus production, CRE Acceptor (MultiBac); Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_206266 Copy
Species: Homo sapiens
Genetic Insert: H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Recombinant baculovirus production; Bacterial Resistance:Gentamicin
References:
Comments: L224Q and A247S mutations in mKO2-hCdt1 will not affect plasmid function.
Proper citation: RRID:Addgene_206280 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2545; Vector Backbone:BASIC_SEVA_69.11; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_194214 Copy
Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: Replicating ORBIT V1 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205291 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Diatom Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_194645 Copy
Species: Scytonema hofmannii
Genetic Insert: ShTnsB, ShTnsC, ShTniQ, ShCas12k
Vector Backbone Description: Backbone Size:3359; Vector Backbone:pR01600; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: The vector backbone was derived from plasmid pFlpe2 from
Choi, K. H., Mima, T., Casart, Y., Rholl, D., Kumar, A., Beacham, I. R., & Schweizer, H. P. (2008). Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Applied and environmental microbiology, 74(4), 1064–1075. https://doi.org/10.1128/AEM.02430-07
Proper citation: RRID:Addgene_180076 Copy
Species:
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Addgene #25752; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_136519 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
