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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCRISPomyces-1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_61736 | sSpCas9 | Synthetic | Apramycin | PMID:25458909 | Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin | 2026-08-08 01:19:31 | 1 | ||
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pCRISPomyces-2 Resource Report Resource Website 10+ mentions |
RRID:Addgene_61737 | sSpCas9 | Synthetic | Apramycin | PMID:25458909 | Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin | 2026-08-08 01:19:31 | 19 | ||
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pKCcas9dO Resource Report Resource Website 1+ mentions |
RRID:Addgene_62552 | Scocas9 | artificially synthesized, codon optimized gene from Strep pyogenes | Apramycin | PMID:25739462 | More information is provided in the supplemental files. Please note that the sequence of the backbone (pKC1139) was obtained via Sanger sequencing and several regions may have discrepancies due to high GC content. These discrepancies have no functional consequences. | Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR, Bacterial Genome Editing; Bacterial Resistance:Apramycin | 2026-08-08 01:19:38 | 4 | |
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pGM1202 Resource Report Resource Website 1+ mentions |
RRID:Addgene_69615 | aac | E. coli | Apramycin | PMID:30191290 | Backbone Size:2140; Vector Backbone:pSG5; Vector Types:; Bacterial Resistance:Apramycin | 2026-08-08 01:20:40 | 2 | ||
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pGM1190 Resource Report Resource Website 1+ mentions |
RRID:Addgene_69994 | aac | E. coli | Apramycin | PMID:30191290 | Backbone Size:2140; Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | 2026-08-08 01:20:44 | 2 | ||
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pJEC726 Resource Report Resource Website |
RRID:Addgene_190814 | dCas9 | Synthetic | Apramycin | PMID:35801861 | Backbone Marker:Addgene; Backbone Size:6573; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin | S. pyogenes dCas9 codon optimized for Streptomyces expression. The D10A and H840A mutations were introduced to convert Cas9 to dCas9. | 2026-08-08 01:25:26 | 0 | |
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pJEC819 Resource Report Resource Website |
RRID:Addgene_190815 | dCas9-XTEN-rpoA | Synthetic | Apramycin | PMID:35801861 | Backbone Size:6578; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin | 2026-08-08 01:25:26 | 0 | ||
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pSelAct-Express Resource Report Resource Website |
RRID:Addgene_174375 | Apramycin | PMID:34890250 | For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning). Original pSelAct backbone was developed here: Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151 pSelAct-Express was develop and tested here: Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345 | Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Expression vector for targeted integration; Bacterial Resistance:Apramycin | 2026-08-08 01:25:14 | 0 | |||
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pEA02 Resource Report Resource Website |
RRID:Addgene_172193 | attL | Synthetic | Apramycin | PMID:35456877 | Backbone Marker:Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.; Backbone Size:3469; Vector Backbone:pOJ260; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | 2026-08-08 01:25:16 | 0 | ||
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pCRISPomyces-FnCpf1 Resource Report Resource Website |
RRID:Addgene_129554 | FnCpf1 | Other | Apramycin | PMID:31090220 | Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | 2026-08-08 01:07:32 | 0 | ||
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pCRISPomyces-Sth1Cas9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_129552 | Sth1Cas9 | Other | Apramycin | PMID:31090220 | Please note that the Addgene verified sequence differs from the depositor's reference sequence. These differences do not affect plasmid function. | Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | 2026-08-08 01:07:32 | 1 | |
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pCRISPomyces-SaCas9 Resource Report Resource Website |
RRID:Addgene_129553 | SaCas9 | Other | Apramycin | PMID:31090220 | Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin | 2026-08-08 01:07:32 | 0 | ||
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pMSR:MSMEG_5121:Dendra Resource Report Resource Website |
RRID:Addgene_167388 | MSMEG_5121 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-08-08 01:11:55 | 0 | |
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pMSR:MSMEG_5114:Dendra Resource Report Resource Website |
RRID:Addgene_167387 | MSMEG_5114 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-08-08 01:11:55 | 0 | |
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pMSR:MSMEG_5103:Dendra Resource Report Resource Website |
RRID:Addgene_167385 | MSMEG_5103 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-08-08 01:11:55 | 0 | |
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pMSR:MSMEG_5060:Dendra Resource Report Resource Website |
RRID:Addgene_167371 | MSMEG_5060 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-08-08 01:11:55 | 0 | |
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pMSR:MSMEG_5059:Dendra Resource Report Resource Website |
RRID:Addgene_167370 | MSMEG_5059 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-08-08 01:11:55 | 0 | |
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pMSR:MSMEG_5070:Dendra Resource Report Resource Website |
RRID:Addgene_167375 | MSMEG_5070 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-08-08 01:11:55 | 0 | |
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pMSR:MSMEG_5068:Dendra Resource Report Resource Website |
RRID:Addgene_167373 | MSMEG_5068 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-08-08 01:11:55 | 0 | |
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pMSR:MSMEG_5061:Dendra Resource Report Resource Website |
RRID:Addgene_167372 | MSMEG_5061 | M. smegmatis | Apramycin | PMID:33653882 | 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) | Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin | 2026-08-08 01:11:55 | 0 |
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