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  • Bacterial Resistance:apramycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCRISPomyces-1
 
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1+ mentions
RRID:Addgene_61736 sSpCas9 Synthetic Apramycin PMID:25458909 Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin 2026-08-08 01:19:31 1
pCRISPomyces-2
 
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10+ mentions
RRID:Addgene_61737 sSpCas9 Synthetic Apramycin PMID:25458909 Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin 2026-08-08 01:19:31 19
pKCcas9dO
 
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1+ mentions
RRID:Addgene_62552 Scocas9 artificially synthesized, codon optimized gene from Strep pyogenes Apramycin PMID:25739462 More information is provided in the supplemental files. Please note that the sequence of the backbone (pKC1139) was obtained via Sanger sequencing and several regions may have discrepancies due to high GC content. These discrepancies have no functional consequences. Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR, Bacterial Genome Editing; Bacterial Resistance:Apramycin 2026-08-08 01:19:38 4
pGM1202
 
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1+ mentions
RRID:Addgene_69615 aac E. coli Apramycin PMID:30191290 Backbone Size:2140; Vector Backbone:pSG5; Vector Types:; Bacterial Resistance:Apramycin 2026-08-08 01:20:40 2
pGM1190
 
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1+ mentions
RRID:Addgene_69994 aac E. coli Apramycin PMID:30191290 Backbone Size:2140; Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-08 01:20:44 2
pJEC726
 
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RRID:Addgene_190814 dCas9 Synthetic Apramycin PMID:35801861 Backbone Marker:Addgene; Backbone Size:6573; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin S. pyogenes dCas9 codon optimized for Streptomyces expression. The D10A and H840A mutations were introduced to convert Cas9 to dCas9. 2026-08-08 01:25:26 0
pJEC819
 
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RRID:Addgene_190815 dCas9-XTEN-rpoA Synthetic Apramycin PMID:35801861 Backbone Size:6578; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin 2026-08-08 01:25:26 0
pSelAct-Express
 
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RRID:Addgene_174375 Apramycin PMID:34890250 For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning). Original pSelAct backbone was developed here: Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151 pSelAct-Express was develop and tested here: Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345 Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Expression vector for targeted integration; Bacterial Resistance:Apramycin 2026-08-08 01:25:14 0
pEA02
 
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RRID:Addgene_172193 attL Synthetic Apramycin PMID:35456877 Backbone Marker:Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.; Backbone Size:3469; Vector Backbone:pOJ260; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-08 01:25:16 0
pCRISPomyces-FnCpf1
 
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RRID:Addgene_129554 FnCpf1 Other Apramycin PMID:31090220 Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-08 01:07:32 0
pCRISPomyces-Sth1Cas9
 
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1+ mentions
RRID:Addgene_129552 Sth1Cas9 Other Apramycin PMID:31090220 Please note that the Addgene verified sequence differs from the depositor's reference sequence. These differences do not affect plasmid function. Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-08 01:07:32 1
pCRISPomyces-SaCas9
 
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RRID:Addgene_129553 SaCas9 Other Apramycin PMID:31090220 Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-08 01:07:32 0
pMSR:MSMEG_5121:Dendra
 
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RRID:Addgene_167388 MSMEG_5121 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-08 01:11:55 0
pMSR:MSMEG_5114:Dendra
 
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RRID:Addgene_167387 MSMEG_5114 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-08 01:11:55 0
pMSR:MSMEG_5103:Dendra
 
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RRID:Addgene_167385 MSMEG_5103 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-08 01:11:55 0
pMSR:MSMEG_5060:Dendra
 
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RRID:Addgene_167371 MSMEG_5060 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-08 01:11:55 0
pMSR:MSMEG_5059:Dendra
 
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RRID:Addgene_167370 MSMEG_5059 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-08 01:11:55 0
pMSR:MSMEG_5070:Dendra
 
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RRID:Addgene_167375 MSMEG_5070 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-08 01:11:55 0
pMSR:MSMEG_5068:Dendra
 
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RRID:Addgene_167373 MSMEG_5068 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-08 01:11:55 0
pMSR:MSMEG_5061:Dendra
 
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RRID:Addgene_167372 MSMEG_5061 M. smegmatis Apramycin PMID:33653882 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed) Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin 2026-08-08 01:11:55 0

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