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Species: Synthetic
Genetic Insert: polyhistidine (6x) tag
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54447 Copy
Species: Synthetic
Genetic Insert: destablized GFP
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54454 Copy
Species: Synthetic
Genetic Insert: 16 aa GS linker + superfolder GFP 1-10 domain
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments: Please note this plasmid contains super-folder GFP and shows noticeable background fluorescence.
Proper citation: RRID:Addgene_54452 Copy
Species: Synthetic
Genetic Insert: c-MYC epitope tag (EQKLISEEDL)
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54450 Copy
Species: Other
Genetic Insert: ccdB A-B
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54459 Copy
Species: Other
Genetic Insert: lacZ C-D
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54458 Copy
Species: Synthetic
Genetic Insert: hygromycin phosphotranferase (hptII) (pCAMBIA)
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54463 Copy
Species: Synthetic
Genetic Insert: superfolder GFP 1-10 domain
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
References:
Comments: Please note this plasmid contains super-folder GFP and shows noticeable background fluorescence.
Proper citation: RRID:Addgene_54461 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC18T-mini-Tn7T-oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_63121 Copy
Species: Mus musculus
Genetic Insert: BICD2N (1-400)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pOmniBac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_64070 Copy
Species:
Genetic Insert: oriR6K
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Genbank Accession #AY884833
Proper citation: RRID:Addgene_64966 Copy
Species:
Genetic Insert: FRT-GmR-FRT
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession #AY597270
Compared to the Genbank reference sequence AY597270, Addgene's sequencing results found a R98K mutation in the gentamycin cassette. The effect of this difference is unknown.
Proper citation: RRID:Addgene_64949 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Beckwith Lab; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_65098 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession # DQ296496
Compared to the Genbank reference sequence DQ296496, Addgene's sequencing results found T920I, D999E and F1000L mutations in lacZ. The effect of these differences is unknown. Note that the amino acid numbering is relative to the lacZ encoded by this plasmid.
Proper citation: RRID:Addgene_65028 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18R6K-mini-Tn7T; Vector Types:; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession # AY599228
Proper citation: RRID:Addgene_65022 Copy
Species:
Genetic Insert: ecfp
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession # DQ493878
Proper citation: RRID:Addgene_65030 Copy
Species: Homo sapiens
Genetic Insert: GATA Binding Protein 6
Vector Backbone Description: Backbone Marker:Addgene#25752; Backbone Size:4422; Vector Backbone:pEN_TTmiRC2; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_72922 Copy
Species:
Genetic Insert: rfp
Vector Backbone Description: Vector Backbone:pRIM2; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_74073 Copy
Species: Synthetic
Genetic Insert: aacC1
Vector Backbone Description: Backbone Marker:Alexeyev, M.F., BioTechniques 18:52-54, 1995; Vector Backbone:pBSL15; Vector Types:Bacterial vector based on pMB1 replicon; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_74106 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: See J. Microbio. Methods 2015 118:75-79 for details
The authors used this vector along with a Tn7 transposase encoding plasmid, pTNS1.
Proper citation: RRID:Addgene_70115 Copy
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