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pRK5-mFzd2
 
Resource Report
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1+ mentions 		RRID:Addgene_42254 Frizzled 2 Mus musculus Ampicillin PMID:23095888 Fzd2 insert contains S193A compared with NM_020510.2. Mutation has no effect on plasmid function Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 2
pRK5-mFzd4
 
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1+ mentions 		RRID:Addgene_42256 Frizzled 4 Mus musculus Ampicillin PMID:23095888 Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 3
pRK5-mFzd6
 
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1+ mentions 		RRID:Addgene_42258 Frizzled 6 Mus musculus Ampicillin PMID:23095888 Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 3
DR274
 
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100+ mentions 		RRID:Addgene_42250 Kanamycin PMID:23360964 Backbone Marker:Joung lab DR274; Backbone Size:2147; Vector Backbone:Zebrafish-gRNA-ExpressionVector; Vector Types:Worm Expression, CRISPR, zebrafish expression; Bacterial Resistance:Kanamycin 2026-07-25 12:46:59 211
pCS6-mPSD95a-TS2-HA
 
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1+ mentions 		RRID:Addgene_42240 PSD95 Ampicillin PMID:23103964 Vector Backbone:pCS6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 2
pENTR-3xFLAG-YAP1-S127A
 
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1+ mentions 		RRID:Addgene_42239 YAP1 Homo sapiens Kanamycin PMID:22308401 Backbone Marker:Invitrogen; Backbone Size:2300; Vector Backbone:pEntr3c; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin Serine 127 changed to alanine 2026-07-25 12:46:59 1
pcDNA3 βarr1 S412D Flag
 
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1+ mentions 		RRID:Addgene_42196 β-arrestin 1 S412D Rattus norvegicus Ampicillin PMID:9924018 Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing. Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S412D 2026-07-25 12:46:58 2
Fz4-GFP
 
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1+ mentions 		RRID:Addgene_42197 Frizzled 4 Mus musculus Kanamycin PMID:12958364 Fz4-GFP was generated by fusing EGFP (Clontech) at its C-terminus. Alternate plasmid names: pEGFPN2 mFz4; pEGFP-N2-Frizzled4 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:46:58 3
pX330-U6-Chimeric_BB-CBh-hSpCas9
 
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1000+ mentions 		RRID:Addgene_42230 humanized S. pyogenes Cas9 Ampicillin PMID:23287718 Alternate plasmid name: pSpCas9(BB) (PX330) For plasmid usage, please see the associated publication (Cong et al. Science. 2013, PMID: 23287718), as well as Ran et al. Nat Protoc. 2013, PMID: 24157548. For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/ Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 1906
pMJ920
 
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10+ mentions 		RRID:Addgene_42234 Cas9 Synthetic Ampicillin PMID:23386978 Backbone Marker:QB3 MacroLab; Backbone Size:6145; Vector Backbone:MacroLab 6D; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin codon-optimized synthetic DNA sequence 2026-07-25 12:46:59 41
pCS6-mPSD95a-TSa-HA
 
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1+ mentions 		RRID:Addgene_42235 PSD95 Ampicillin PMID:20155731 Vector Backbone:pCS6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 2
Tol2-zTRAP
 
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1+ mentions 		RRID:Addgene_42236 EGFP-Rpl10a Danio rerio Ampicillin PMID:23281262 Backbone Size:6170; Vector Backbone:pT2KXIG; Vector Types:; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 1
pMyC
 
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1+ mentions 		RRID:Addgene_42192 Hygromycin PMID:33006405 Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München) Backbone Marker:Daugelat et al 2003; Backbone Size:6900; Vector Backbone:pSD26; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-07-25 12:46:58 6
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
 
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10+ mentions 		RRID:Addgene_42229 humanized S. pyogenes Cas9 Ampicillin PMID:23287718 Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function. For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/ Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 36
pMQ430
 
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1+ mentions 		RRID:Addgene_42216 dam E. coli K-12 Ampicillin PMID:12897023 Vector Backbone:pBAD18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 1
pcDNA3-GFP-IMP2-2_antisense-control
 
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1+ mentions 		RRID:Addgene_42174 Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control Homo sapiens Ampicillin PMID:23257922 Backbone Marker:invitrogen; Backbone Size:6157; Vector Backbone:pcDNA3.1/CT-GFP TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:46:58 2
pC1-HyPer-C199S
 
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1+ mentions 		RRID:Addgene_42213 HYPER C199S Synthetic Kanamycin PMID:16554833 Backbone Size:3982; Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:46:59 8
pRK5-mWnt16
 
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1+ mentions 		RRID:Addgene_42291 Wnt16 Mus musculus Ampicillin PMID:23095888 Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:46:59 1
pcDNA3 c-SRC (151-536)
 
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1+ mentions 		RRID:Addgene_42207 c-SRC (151-536) Homo sapiens Ampicillin PMID:10753943 All c-SRC mutants were generated by polymerase chain reaction using c-SRC (WT) (Addgene Plasmid #42202) as a template, and polymerase chain reaction products containing a minimal Kozak sequence were cloned into the HindIII and XhoI sites of pcDNA3. This construct contains amino acids 151-536 of human c-SRC and is untagged. All sequences were confirmed using an automated ABI DNA sequencer (Howard Hughes Nucleic Acid Facility, Duke University). This plasmid also contains an L176F point mutation which should not affect function. Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin contains amino acids 151-536 2026-07-25 12:46:58 2
pcDNA3 c-SRC (250-536)
 
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1+ mentions 		RRID:Addgene_42208 c-SRC (250-536) Homo sapiens Ampicillin PMID:10753943 All c-SRC mutants were generated by polymerase chain reaction using c-SRC (WT) (Addgene Plasmid #42202) as a template, and polymerase chain reaction products containing a minimal Kozak sequence were cloned into the HindIII and XhoI sites of pcDNA3. This construct contains amino acids 250-536 of human c-SRC and is untagged. All sequences were confirmed using an automated ABI DNA sequencer (Howard Hughes Nucleic Acid Facility, Duke University). Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin contains amino acids 250-536 2026-07-25 12:46:58 2

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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."

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