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Species: M. smegmatis
Genetic Insert: MSMEG_0392
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159060 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0385
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159058 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0389
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159059 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0372
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159056 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0371
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159055 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0311
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159052 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0635
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159072 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0622
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159071 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0576
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159067 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0581
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159068 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0758
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159083 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0760
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159084 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0754
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159082 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0736
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159080 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0732
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159079 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0690
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159076 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0678
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
References:
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159074 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pJMD13; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_138653 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin
References:
Comments: Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3).
1. First development of pSelAct for knockouts in Rhodococcus equi:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans:
Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022
3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174374 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Size:3896; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_174517 Copy
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