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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
HL 5226
 
Resource Report
Resource Website
RRID:Addgene_63667 see comments None PMID:26261213 MG1655 + lacIq intergrated at intS + ΔglmZ Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-07-25 12:49:53 0
SJ358
 
Resource Report
Resource Website
RRID:Addgene_67755 none None PMID:26251500 The genotype of the strain relative to MG1655 is complex, since there are many single nucleotide variations and several 10-20kb differences (Brown & Jun (2015) Genome Announcements, Lyons et. al. (2011) PLoS ONE). There are a particular abundance of differences in the rDNA operons, which in several cases align more closely to E. coli strains DH10B, MDS42, and W. Particularly relevant genotypes are: λ+ gal+ eut+ pyrE+ ilvG+ rpoS33Am glnV(SupAm) evgA::IS1 Δrfb Vector Backbone:none; Vector Types:; Bacterial Resistance:None 2026-07-25 12:50:29 0
MG1655 Z1 malE
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_65915 MG1655 Z1 malE None PMID:26900850 F-, lambda-, rph-1, laciq, PN25-tetR, SpR, malE- Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None 2026-07-25 12:50:13 1
CY027
 
Resource Report
Resource Website
RRID:Addgene_72402 None PMID:26315440 No antibiotic resistance. Vector Backbone:E. coli BW25113; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:None 2026-07-25 12:51:07 0
HL 6703
 
Resource Report
Resource Website
RRID:Addgene_69778 None PMID:27084942 Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-07-25 12:50:44 0
HL 6686
 
Resource Report
Resource Website
RRID:Addgene_69775 None PMID:27084942 Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-07-25 12:50:44 0
HL 3500
 
Resource Report
Resource Website
RRID:Addgene_69774 None PMID:27084942 Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-07-25 12:50:44 0
HL 6778
 
Resource Report
Resource Website
RRID:Addgene_69782 None PMID:27084942 Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-07-25 12:50:44 0
HL 6777
 
Resource Report
Resource Website
RRID:Addgene_69781 None PMID:27084942 Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-07-25 12:50:44 0
BLIM cells
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_35609 none None PMID:10610690 To be used with the following plasmids from the Matthews lab: pTARA (www.addgene.org/31491), pLS1 (www.addgene.org/31490), and (www.addgene.org/31492) pLS1/-11 Vector Backbone:NA; Vector Types:; Bacterial Resistance:None 2026-07-25 12:46:10 1
LC-E03
 
Resource Report
Resource Website
RRID:Addgene_78552 pTet--Cas9 cassette integrated at 186 primary attB site None PMID:27060147 Vector Backbone:na; Vector Types:; Bacterial Resistance:None This is a bacterial strain 2026-07-25 12:52:07 0
pSELECT-HA-mFOXO1-D256
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_83379 Forkhead box O1 Mus musculus None Backbone Marker:Invivogen; Backbone Size:3390; Vector Backbone:pSELECT-puro; Vector Types:Mammalian Expression; Bacterial Resistance:None N256TAG (premature stop codon) 2026-07-25 12:52:46 1
E. coli ER1821ΔlacI
 
Resource Report
Resource Website
RRID:Addgene_141407 None PMID:31980824 λ- F- glnX44 e14- (McrA-) rfbD1 endA1 thi-1 Δ(yjiT-opgB)114::IS10 (EcoKI R- M- McrBC- Mrr-) + rpoS393(am) creC510 lrhA::IS3 ydeN::IS10 ΔlacI Verification of lacI deletion: PCR reaction on genomic DNA using AK362 and AK365 primers produces a 1936 bp fragment AK 362 5’-CAATACCAATCGCACGCGG AK 365 5’-CGAGACGTCACGGAAAATGCC Phenotype: Constitutive β-galactosidase synthesis This strain was derived from the precursor strain, E. coli ER1821, which is described in Jobling et al. (2016) Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems. Genome Announc 4(4):e00763-16. https://www.ncbi.nlm.nih.gov/pubmed/27516504 Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None 2026-07-25 12:39:41 0
LC-E18
 
Resource Report
Resource Website
RRID:Addgene_115924 none None PMID:29765036 E. coli K12 MG1655 genotype: F- λ- ilvG- rfb-50 rph-1 Integration at lambda attB: pOSIP-KL-sulA-GFP Integration at HK022 attB: pOSIP-KO-RBS2-dCas9 SulA-GFP acts as an SOS response reporter. Vector Backbone:none; Vector Types:; Bacterial Resistance:None 2026-07-25 12:35:53 0
AV04
 
Resource Report
Resource Website
RRID:Addgene_115926 none None PMID:29765036 E. coli K12 MG1655 genotype: F- λ- ilvG- rfb-50 rph-1 Integration at lambda attB: pOSIP-KL-mCherry Integration at primary 186 attB: pOSIP-KO-RBS2-dCas9 mCherry quantifies dCas9 repression Vector Backbone:none; Vector Types:; Bacterial Resistance:None 2026-07-25 12:35:53 0
IF189
 
Resource Report
Resource Website
RRID:Addgene_134836 lysogen of a temperature-inducible bacteriophage lambda None PMID:28522818 Genotype is [λ+ Lac- galK2 IN(rrnD-rrnE)1 rph-1], a derivative of strain W3102 Phenotypic assay: Grow at 30°C, but restricted at 42°C due to the induction of phage particles and cell lysis. Vector Backbone:none; Vector Types:; Bacterial Resistance:None 2026-07-25 12:38:46 0
E. coli BL21 (DE3) ΔEntD
 
Resource Report
Resource Website
RRID:Addgene_192874 The strain has a 535 nt deletion between nt 15 and nt 551 in the EntD gene. E.coli None PMID:16709676 EntD, a 4'-phosphopantetheinyl transferase, is required for native enterobactin synthesis. This strain allows for expression of nonribosomal peptide synthetase (NRPS) carrier proteins that do not harbor a phosphopantetheinyl group. Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None 2026-07-25 12:58:47 0
Δrpi
 
Resource Report
Resource Website
RRID:Addgene_234837 ΔrpiAB Δedd strain None Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None 2026-07-25 01:04:11 0
B95 ΔAΔfabRΔselABC
 
Resource Report
Resource Website
RRID:Addgene_234550 None None PMID:34411545 Genotype: The same as BL21(DE3) except for the additional mutations at 95 UAG codons, disruption of prfA, and frameshift in fabR and deletion of selABC. Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None 2026-07-25 01:03:34 0
TB205 △proC △hisL
 
Resource Report
Resource Website
RRID:Addgene_229552 MG1655 attP21::PR-mCherry::frt proC::frt hisL::frt n/a None PMID:40509754 Primers for sequence verification of hisL knock-out: Fwd - prEP229, gtgaggataagaggtggcga Rev - prEP230, tcctttccccgctcattcat Please visit https://doi.org/10.1101/2024.07.19.604250 for bioRxiv preprint. Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None 2026-07-25 01:03:48 0

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