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PresentER-SIINFEKL (mCherry)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_102945 SIINFEKL Gallus gallus Ampicillin PMID:30499773 Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint. Backbone Size:8200; Vector Backbone:PresentER; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin OVA257-264 2026-07-25 12:33:52 4
pHMR1A
 
Resource Report
Resource Website 		RRID:Addgene_102957 MR1A cDNA Homo sapiens Ampicillin PMID:24832684 Backbone Marker:Invitrogen; Backbone Size:3908; Vector Backbone:pCR2.1-TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin Stop codon has been removed by HindIII site 2026-07-25 12:33:52 0
pMin1
 
Resource Report
Resource Website 		RRID:Addgene_102959 Tetracycline PMID:8566811 Artificial DNA for plasmid pMin1 GenBank: Z50148.1 Backbone Size:2026; Vector Backbone:pRH144; Vector Types:mini-transposon system suitable for sequencing, shuttling and gene fusions; Bacterial Resistance:Tetracycline 2026-07-25 12:33:52 0
pET22b_PfSSB_W166C
 
Resource Report
Resource Website 		RRID:Addgene_103001 PfSSB_W166C Plasmodium falciparum Ampicillin PMID:29466468 Usage: The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, with two point mutations, C93A and W166C, for subsequent labeling at the 166 cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) or Cy3B per subunit. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77.W166C is the equivalent position to W88 in E. coli SSB, which was labeled with Cy3B for single molecule measurements of DNA unwinding using TIRF (Fili, N.; Mashanov, G.; Toseland, C. P.; Batters, C.; Wallace, M. I.; Yeeles, J. T. P.; Dillingham, M. S.; Webb, M. R.; Molloy, J. E., Visualizing DNA unwinding by helicases at the single molecule level. Nucleic Acids Res. 2010, 38, 4448-4457.) Protocol: See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233. Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C93A, W166C 2026-07-25 12:33:53 0
pET22b_PfSSB
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_103000 PfSSB Plasmodium falciparum Ampicillin PMID:29466468 The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, for subsequent labeling at the single, native cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~20-fold fluorescence increase on binding ssDNA with very high affinity. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77. Protocol See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233. Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 1
pUCmini-iCAP-PHP.B2
 
Resource Report
Resource Website 		RRID:Addgene_103003 Synthetic construct isolate AAV-PHP.B2 VP1 gene Synthetic Ampicillin PMID:26829320 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 0
pUCmini-iCAP-PHP.B
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_103002 Synthetic construct isolate AAV-PHP.B VP1 gene Synthetic Ampicillin PMID:26829320 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 12
pUCmini-iCAP-PHP.eB
 
Resource Report
Resource Website
100+ mentions 		RRID:Addgene_103005 Synthetic construct isolate AAV-PHP.eB VP1 gene Synthetic Ampicillin PMID:28671695 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 121
pUCmini-iCAP-PHP.B3
 
Resource Report
Resource Website 		RRID:Addgene_103004 Synthetic construct isolate AAV-PHP.B3 VP1 gene Synthetic Ampicillin PMID:26829320 Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. *This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction. Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 0
pJH124
 
Resource Report
Resource Website 		RRID:Addgene_102951 APEX2-NES Saccharomyces cerevisiae Ampicillin PMID:27274088 Backbone Marker:ATCC; Backbone Size:7500; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:33:52 0
pZL1-ZF - myelin basic protein a (mbpa)
 
Resource Report
Resource Website 		RRID:Addgene_103007 myelin basic protein Danio rerio Ampicillin PMID:12112375 Backbone Size:4308; Vector Backbone:pZL1; Vector Types:; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 0
GESTALT_pX330-v1
 
Resource Report
Resource Website 		RRID:Addgene_103061 integration of Cas9 with guide targeting GESTALT barcodes V1 to V5 Homo sapiens Ampicillin PMID:27229144 Derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 Vector Backbone:lentiCRISPR v2 (#52961); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 0
pCAGGS_3E5E_luciferase
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_103055 Ebolavirus minigenome Zaire ebolavirus, Mayinga variant Ampicillin PMID:28807685 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 2
pSC101_TIMER
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_103057 TIMER-bac Synthetic Kanamycin PMID:25126781 TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance. Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin mutations: S4T and S179T 2026-07-25 12:33:53 4
pCAGGS_L_EBOV
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_103052 L gene of Ebola virus Zaire ebolavirus, Mayinga Ampicillin PMID:28807685 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 4
pCAGGS_VP30_EBOV
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_103051 VP30 gene of Ebola virus Zaire ebolavirus, Mayinga Ampicillin PMID:28807685 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 3
pCAGGS_HA-VP35_EBOV
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_103053 VP35 Zaire ebolavirus, Mayinga Ampicillin PMID:27252530 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 1
pNB22
 
Resource Report
Resource Website 		RRID:Addgene_103059 GFP-OVA Other Ampicillin PMID:24439899 A short ovalbumin epitope (amino acids 319–343) was fused to GFP (pGFP_OVA) to follow the immune response to the expressed antigen. (Bumann, 2001). Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 0
pUC19-B9M9X8
 
Resource Report
Resource Website 		RRID:Addgene_103072 B9M9X8(Cas9 coding gene from Acidovorax ebreus (strain TPSY)) Other Ampicillin PMID:29529247 Backbone Marker:NEB(New England Biolabs); Vector Backbone:pUC19; Vector Types:CRISPR, Cloning vector; Bacterial Resistance:Ampicillin human codon-optimized 2026-07-25 12:33:53 0
pKSJ331
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_103063 Colicin E1 and E1 immunity protein E. coli Ampicillin PMID:19029376 Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene. Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:33:53 1

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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."

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