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Species: Synthetic
Genetic Insert: SSPB(micro)-mVenus-ppKin14VIb(861-1321)
Vector Backbone Description: Vector Backbone:B-actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32328628
Comments: Internal plasmid ID: WN277. Phytozome gene ID: Pp3c9_4530
Proper citation: RRID:Addgene_174641 Copy
Species: Mus musculus
Genetic Insert: Meikin
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM606, compatible with in vitro transcription from SP6 promoter . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174710 Copy
Species: Homo sapiens
Genetic Insert: PARP1-K893I
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, K893I) (see PMID: 8473297 for detail about the mutation)
Proper citation: RRID:Addgene_174799 Copy
Species: Mus musculus
Genetic Insert: Meikin
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM963, compatible with in vitro transcription from SP6 promoter, C-terminal Separase cleavage site is derived from human Rad21 (aa142-275) with Separase resistant mutations (E169R, R172E), kinetochore localization is maintainted by C-terminal mMeikin fragment (aa387-434) . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174713 Copy
Species: Homo sapiens
Genetic Insert: Meikin
Vector Backbone Description: Vector Backbone:pBABE-Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM848 . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174714 Copy
Species: Homo sapiens
Genetic Insert: Meikin
Vector Backbone Description: Vector Backbone:pBABE-Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM849 . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174715 Copy
Species: Homo sapiens
Genetic Insert: Rec8
Vector Backbone Description: Vector Backbone:pBABE-Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM869, Rec8 is fused to human Meikin fragment containing Separase-resistant mutations (aa1-332, E151R, R154E) . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174716 Copy
Species: Homo sapiens
Genetic Insert: Rec8
Vector Backbone Description: Vector Backbone:pBABE-Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM937 . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174717 Copy
Species: Homo sapiens
Genetic Insert: PARP1-W318R
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (W318R, V762A) (see PMID: 26626480 for detail about the mutation)
Proper citation: RRID:Addgene_174793 Copy
Species: Homo sapiens
Genetic Insert: PARP1-A762V
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (see PMID: 20399804 for detail about the mutation)
Proper citation: RRID:Addgene_174795 Copy
Species: Homo sapiens
Genetic Insert: PARP1-A774L
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, A774L) (*protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol) (see PMID: 26626480 for detail about the mutation)
Proper citation: RRID:Addgene_174796 Copy
Species: Homo sapiens
Genetic Insert: PARP1-A870L
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, A870L) (*protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol) (see PMID: 26626480 for detail about the mutation)
Proper citation: RRID:Addgene_174797 Copy
Species: Homo sapiens
Genetic Insert: Meikin
Vector Backbone Description: Vector Backbone:AAVS1-TRE3G-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM604, contains homology arms for HDR integration into human AAVS1 locus . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174707 Copy
Species: Mus musculus
Genetic Insert: Meikin
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM530, compatible with in vitro transcription from SP6 promoter . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174708 Copy
Species: Homo sapiens
Genetic Insert: Lenti-evoA1-BE4max
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34663942
Proper citation: RRID:Addgene_174700 Copy
Species: Homo sapiens
Genetic Insert: Lenti-evoA1-seBEN
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34663942
Comments: 5' Cloning Site: BsmbI (unknown if destroyed), 3' Cloning Site: BsmbI (unknown if destroyed).
Proper citation: RRID:Addgene_174701 Copy
Species: Mus musculus
Genetic Insert: mouse MLH1dn (codon optimized)
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34653350
Proper citation: RRID:Addgene_174825 Copy
Species: Homo sapiens
Genetic Insert: Meikin
Vector Backbone Description: Vector Backbone:AAVS1-TRE3G-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM603, contains homology arms for HDR integration into human AAVS1 locus . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174706 Copy
Species: X. perforans
Genetic Insert: GG_XopJ4, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174783 Copy
Species: N. benthamiana
Genetic Insert: GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174785 Copy
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