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Species: Synthetic
Genetic Insert: 6xHIS uncleavable
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174589 Copy
Vector Backbone Description: Vector Backbone:pOPIN-A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_174581 Copy
Species: Synthetic
Genetic Insert: Twin Streptavidin uncleavable
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174586 Copy
Species: Synthetic
Genetic Insert: 6xHIS-GB1
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174587 Copy
Species: Synthetic
Genetic Insert: attP and 3xP3-GFP-SV40 BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: attP site within the fluorescence marker cassette is to allow subsequent insertions of attB plasmids in the knock-in locus
Proper citation: RRID:Addgene_174534 Copy
Species: Synthetic
Genetic Insert: PUb-mTurquoise BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: The added N-terminal NLS in mTurquoise does not work very well, fluorescence is mostly cytoplasmic
Proper citation: RRID:Addgene_174535 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174495 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27473911
Comments: For protein expression, grow at 17degC.
Plasmid used in the following publication:
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M
J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.
Proper citation: RRID:Addgene_174496 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Vector Backbone:pnEA-vH; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174497 Copy
Species: Synthetic
Genetic Insert: 3xP3-GFP BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: The Tub56D terminator was chosen as an alternative to SV40
Proper citation: RRID:Addgene_174531 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN6
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174499 Copy
Species: Synthetic
Genetic Insert: 3xP3-DsRed BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174532 Copy
Species: Synthetic
Genetic Insert: 3xP3-mTurquoise BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174533 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Vector Backbone:pnEA-vH; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174491 Copy
Species: Homo sapiens
Genetic Insert: FECH with yeast HEM15 promoter and terminator
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34517185
Comments: Please note: The plasmid contain 17 nucleotide discrepancies at N-terminus of the FEECH sequence compared to the depositor's provided sequence. The depositor noted that these differences do not affect the plasmid's function.
Proper citation: RRID:Addgene_174524 Copy
Species: Homo sapiens
Genetic Insert: VPS29
Vector Backbone Description: Backbone Size:4700; Vector Backbone:YFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18981234
Proper citation: RRID:Addgene_174520 Copy
Species: Homo sapiens
Genetic Insert: DDA1
Vector Backbone Description: Backbone Size:6785; Vector Backbone:pAC8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31686031
Proper citation: RRID:Addgene_174480 Copy
Species: Moraxella bovoculi AAX11_00205
Genetic Insert: huMb3Cas12a
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Feng Zhang Lab (Addgene #92293) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1
Proper citation: RRID:Addgene_174677 Copy
Species: Butyrivibrio fibrisolvens
Genetic Insert: huBfCas12a
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Feng Zhang Lab (Addgene #92301) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1
Proper citation: RRID:Addgene_174672 Copy
Species: Moraxella bovoculi AAX08_00205
Genetic Insert: huMb2Cas12a
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Feng Zhang Lab (Addgene #92292) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1
Proper citation: RRID:Addgene_174676 Copy
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