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MSCV-IRES-Thy1.1 DEST
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_17442 Ampicillin PMID:16873067 Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors. Backbone Marker:Philippa Marrack Lab; Backbone Size:6063; Vector Backbone:MSCV-IRES-Thy1.1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-01 01:08:36 46
pIVT-dCas9-EGFP
 
Resource Report
Resource Website 		RRID:Addgene_174442 Cas9 Endonuclease Dead Streptococcus pyogenes Ampicillin PMID:34433012 Vector Backbone:pIVT; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin 2026-08-01 01:08:36 0
MSCV-FOXP3-IRES-Thy1.1
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_17443 FOXP3 Homo sapiens Ampicillin PMID:16873067 Backbone Size:6063; Vector Backbone:MSCV-IRES-Thy1.1 DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-01 01:08:36 3
pEGFP-hsp27 wt FL
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_17444 small heat shock protein27 Homo sapiens Kanamycin PMID:17597071 Backbone Marker:BD Biosciences Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 13
pLenti CMV GFP Hygro (656-4)
 
Resource Report
Resource Website
50+ mentions 		RRID:Addgene_17446 GFP Ampicillin PMID:19657394 Backbone Size:8591; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:08:36 57
pLenti CMV GFP Neo (657-2)
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_17447 GFP Ampicillin PMID:19657394 Backbone Size:8068; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:08:36 31
C205
 
Resource Report
Resource Website 		RRID:Addgene_174359 ePAB Xenopus laevis Ampicillin PMID:34213414 Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Multiple silent mutations introduced to be resistant to siRNAs 2026-08-01 01:08:35 0
pFX-GalT-SECFP
 
Resource Report
Resource Website 		RRID:Addgene_174470 SECFP with golgi apparatus-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 0
pFX-mCherry-Sec61β
 
Resource Report
Resource Website 		RRID:Addgene_174468 Venus with endoplasmic reticulum-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 0
pFX-GalT-Sirius
 
Resource Report
Resource Website 		RRID:Addgene_174469 Sirius with golgi apparatus-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 0
pFX-EGFP-Rab11
 
Resource Report
Resource Website 		RRID:Addgene_174460 EGFP with recycling endosome-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 0
pFX-iRFP-Rab11
 
Resource Report
Resource Website 		RRID:Addgene_174462 iRFP with recycling endosome-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 0
pFX-Sirius-Sec61β
 
Resource Report
Resource Website 		RRID:Addgene_174464 Sirius with endoplasmic reticulum-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 0
pFX-SECFP-Sec61β
 
Resource Report
Resource Website 		RRID:Addgene_174465 SECFP with endoplasmic reticulum-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 0
pFX-EGFP-Sec61β
 
Resource Report
Resource Website 		RRID:Addgene_174466 Sirius with endoplasmic reticulum-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 0
pFX-Venus-Sec61β
 
Resource Report
Resource Website 		RRID:Addgene_174467 Venus with endoplasmic reticulum-targeting sequence Other Kanamycin PMID:31735740 Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:36 0
pET-pA-Hia5
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174372 protein A - hia5 Other Kanamycin PMID:35396487 The depositing laboratory recommends Addgene Plasmid 222303: prhaBAD-pA-Hia5, an improved version of this plasmid. Protocol for purification and expression can be found at: dx.doi.org/10.17504/protocols.io.bv82n9ye Application for DiMeLo-seq is described in: doi.org/10.1101/2021.07.06.451383 Protocol was adapted and modified from: doi.org/10.1126/science.aaz1646 Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:08:35 1
pSelAct-KO
 
Resource Report
Resource Website 		RRID:Addgene_174374 Apramycin PMID:34890250 Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3). 1. First development of pSelAct for knockouts in Rhodococcus equi: Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151 2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans: Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022 3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter:  Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345 Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin 2026-08-01 01:08:35 0
pB-CAG-TEVp-IRES-mCherry
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174377 TEV Protease Ampicillin PMID:37053554 Vector Backbone:pB-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:35 1
pAAV-syn-Flpo
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_174378 FLPo recombinase Ampicillin PMID:38212587 Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-01 01:08:35 2

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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."

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