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Species: Homo sapiens
Genetic Insert: Ndel1 shRNA
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:3130; Vector Backbone:pSilencer 2.0-U6; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15473966
Comments: Ndel1 base pair 276-294: GCAGG TCTCA GTGTT AGAA.
Proper citation: RRID:Addgene_12573 Copy
Vector Backbone Description: Backbone Marker:Modified from Clontech's pMSCV; Backbone Size:6800; Vector Backbone:pMSCV-TAP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16919458
Comments: A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5' -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3' and right primer 5' -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3' . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clontech) cut with BglII/EcoRI. See author's map for more information (Note that the NotI site is not precisely underlined in the author's map).
Proper citation: RRID:Addgene_12570 Copy
Species: Homo sapiens
Genetic Insert: ABEmax(TadA E59A TadA* V106F)
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31086823
Proper citation: RRID:Addgene_125652 Copy
Species: Homo sapiens
Genetic Insert: ABEmax(TadA E59A TadA* D108Q)
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31086823
Proper citation: RRID:Addgene_125655 Copy
Species: Homo sapiens
Genetic Insert: ABEmax(TadA E59A TadA* D108M)
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31086823
Proper citation: RRID:Addgene_125656 Copy
Species: Homo sapiens
Genetic Insert: ABEmax(TadA E59A TadA* D108W)
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31086823
Proper citation: RRID:Addgene_125653 Copy
Species: Homo sapiens
Genetic Insert: ABEmax(TadA E59A TadA* R47W)
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31086823
Proper citation: RRID:Addgene_125659 Copy
Species: Homo sapiens
Genetic Insert: ABEmax(TadA E59A TadA* R47Q)
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31086823
Proper citation: RRID:Addgene_125657 Copy
Species: Streptococcus pyogenes
Genetic Insert: Hypa-nCas9n
Vector Backbone Description: Backbone Size:3306; Vector Backbone:pT3TS; Vector Types:RNA in vitro transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32006412
Proper citation: RRID:Addgene_125645 Copy
Species: Homo sapiens
Genetic Insert: ABEmax(TadA E59A)
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31086823
Proper citation: RRID:Addgene_125648 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6799; Vector Backbone:LentiV_Blast; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31697837
Comments: Please visit https://www.biorxiv.org/content/10.1101/636969v1 for BioRxiv preprint
Proper citation: RRID:Addgene_125592 Copy
Vector Backbone Description: Backbone Size:10000; Vector Backbone:LRG2.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31697837
Comments: Please visit https://www.biorxiv.org/content/10.1101/636969v1 for BioRxiv preprint
Proper citation: RRID:Addgene_125593 Copy
Species: Burkholderia pseudomallei
Genetic Insert: BpWcbI
Vector Backbone Description: Backbone Marker:SGC Oxford; Backbone Size:7284; Vector Backbone:pNIC28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25075317
Proper citation: RRID:Addgene_125591 Copy
Vector Backbone Description: Backbone Size:10000; Vector Backbone:LRG2.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31697837
Comments: Please visit https://www.biorxiv.org/content/10.1101/636969v1 for BioRxiv preprint
Proper citation: RRID:Addgene_125594 Copy
Species: Homo sapiens
Genetic Insert: Fos proto-oncogene, AP-1 transcription factor subunit
Vector Backbone Description: Vector Backbone:pFUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517869
Proper citation: RRID:Addgene_125598 Copy
Species: Homo sapiens
Genetic Insert: PPAT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30964683
Proper citation: RRID:Addgene_125620 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
Nevertheless, for sfGFP expression, the depositor recommends the updated versions of this plasmid, PB CAG-sfGFP (https://www.addgene.org/133568/) or PB-CA sfGFP (https://www.addgene.org/159094/), that can also be used without the piggyBac transposase.
Red fluorescent protein versions are also available: PB CAG-tdTomato (https://www.addgene.org/133569/) or PB-CA tdTomato (https://www.addgene.org/159095/).
Proper citation: RRID:Addgene_125747 Copy
Species: Synthetic
Genetic Insert: sfGFP-GSAx9-iCre-ERT2
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Linker between sfGFP and iCre-ERT2 is a clean GSAx9 sequence, similar in length to the mutated P2A sequence in the plasmid pCAG-sfGFP-iCre-ERT2 (#125749).
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). In short, it is not visible to the eyes, but can be detected with flow cytometry.
Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
Proper citation: RRID:Addgene_125748 Copy
Species: Other
Genetic Insert: BE4max-NG
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-auFYCwa9cwpOwF75PnyS for additional information.
Proper citation: RRID:Addgene_125617 Copy
Genetic Insert: PSD95.FingR-eGFP-CCR5TC
Vector Backbone Description: Backbone Size:4589; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32674057
Proper citation: RRID:Addgene_125691 Copy
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
