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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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JM106 Resource Report Resource Website |
RRID:Addgene_49757 | Relevant genotype: endA1, gyrA96, thi, hsdR17, supE44, relA1, λ-, Δ(lac-proAB) | E.coli | None | PMID:2985470 | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:48:01 | 0 | ||
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JM101 Resource Report Resource Website |
RRID:Addgene_50349 | Relevant genotype: supE, thi, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15] | E. coli | None | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:48:04 | 0 | |||
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JM83 Resource Report Resource Website |
RRID:Addgene_50348 | Relevant genotype: ara, Δ(lac-proAB), rspL(+strA), ϕ80, lacZΔM15 | E. coli | None | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:48:04 | 0 | |||
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DH10B-ALT Resource Report Resource Website |
RRID:Addgene_61151 | None | PMID:23479654 | In addition to the wt copy of lacI, another copy of lacI driven by PIq is integrated at the attB Contains another copy of araC driven by a constitutive promoter in addition to wt araC. | Vector Backbone:NA; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:31 | 0 | |||
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HL 1951 Resource Report Resource Website |
RRID:Addgene_61162 | See comments | None | PMID:22833608 | MG1655 + PLlacO-1::T710::yfp intergrated at galK | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-07-25 12:49:31 | 0 | ||
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HL 1852 Resource Report Resource Website |
RRID:Addgene_61161 | See comments | None | PMID:22833608 | HL1745 + PLlacO-1::T710::yfp intergrated at galK | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-07-25 12:49:31 | 0 | ||
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HL 5222 Resource Report Resource Website |
RRID:Addgene_61166 | See comments | None | PMID:22833608 | MG1655 + PLlacO-1::T710::yfp intergrated at yjiP + ∆lacI | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-07-25 12:49:31 | 0 | ||
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HL 5221 Resource Report Resource Website |
RRID:Addgene_61165 | See comments | None | PMID:22833608 | MG1655 + PLlacO-1::T710::yfp intergrated at yfjV + ∆lacI | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-07-25 12:49:31 | 0 | ||
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ML26 Resource Report Resource Website |
RRID:Addgene_61917 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH Precursor strain = ML23 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:37 | 0 | ||
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ML31 Resource Report Resource Website |
RRID:Addgene_61918 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH metA Precursor strain = ML26 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:37 | 0 | ||
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ML21 Resource Report Resource Website |
RRID:Addgene_61914 | none | None | PMID:21925267 | Genotype = tyrA hisG Precursor strain = ML14 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Tyr), His Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:37 | 0 | ||
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ML43 Resource Report Resource Website |
RRID:Addgene_61922 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB Precursor strain = ML42 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys, Thr, Asn *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:37 | 0 | ||
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YM138 Resource Report Resource Website |
RRID:Addgene_61953 | none | None | PMID:26577727 | Genotype = cysE Precursor strain = C43(DE3) Selective amino acid labeling (and/or requirement) = Cys Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:38 | 0 | ||
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MS1 Resource Report Resource Website |
RRID:Addgene_61960 | none | None | PMID:26577727 | Genotype = cysE hisG Precursor strain = YM138 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = Cys, His Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:38 | 0 | ||
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RF1 Resource Report Resource Website |
RRID:Addgene_61962 | none | None | PMID:26577727 | Genotype = glyA Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Gly Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:38 | 0 | ||
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RF4 Resource Report Resource Website |
RRID:Addgene_62072 | none | None | PMID:26577727 | Genotype = aspC tyrB Precursor strain = RF3 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, (Phe) ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:39 | 0 | ||
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RF13 Resource Report Resource Website |
RRID:Addgene_62078 | none | None | PMID:26577727 | Genotype = aspC tyrB trpA trpB Precursor strain = RF4 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, Trp, (Phe) ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:39 | 0 | ||
|
RF6 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62074 | none | None | PMID:26577727 | Genotype = proC Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Pro Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:39 | 1 | ||
|
RF8 Resource Report Resource Website |
RRID:Addgene_62075 | none | None | PMID:26577727 | Genotype = asnA asnB Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asn Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-07-25 12:49:39 | 0 | ||
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HL 3338 Resource Report Resource Website |
RRID:Addgene_60376 | See comments | None | PMID:21189298 | MG1655 + lacIq intergrated at intS + Δ hfq + Δ ryhB | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-07-25 12:49:25 | 0 |
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Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
